Mass spectrometry for proteomics-based investigation Journal Article
| Authors: | Woods, A. G.; Sokolowska, I.; Ngounou Wetie, A. G.; Wormwood, K.; Aslebagh, R.; Patel, S.; Darie, C. C. |
| Article Title: | Mass spectrometry for proteomics-based investigation |
| Abstract: | Within the past years, we have witnessed a great improvement in mass spectrometry (MS) and proteomics approaches in terms of instrumentation, protein fractionation, and bioinformatics. With the current technology, protein identifi cation alone is no longer suffi cient. Both scientists and clinicians want not only to identify proteins but also to identify the protein’s posttranslational modifi cations (PTMs), protein isoforms, protein truncation, protein–protein interaction (PPI), and protein quantitation. Here, we describe the principle of MS and proteomics and strategies to identify proteins, protein’s PTMs, protein isoforms, protein truncation, PPIs, and protein quantitation. We also discuss the strengths and weaknesses within this fi eld. Finally, in our concluding remarks we assess the role of mass spectrometry and proteomics in scientifi c and clinical settings in the near future. This chapter provides an introduction and overview for subsequent chapters that will discuss specifi c MS proteomic methodologies and their application to specifi c medical conditions. Other chapters will also touch upon areas that expand beyond proteomics, such as lipidomics and metabolomics. © 2014 Springer International Publishing Switzerland. |
| Keywords: | protein phosphorylation; nonhuman; binding affinity; mass spectrometry; carboxy terminal sequence; protein protein interaction; protein binding; proteomics; protein processing; amino acid sequence; tandem mass spectrometry; matrix assisted laser desorption ionization time of flight mass spectrometry; protein database; liquid chromatography; ion exchange chromatography; physical chemistry; polyacrylamide gel electrophoresis; affinity chromatography; electrospray mass spectrometry; protein glycosylation; limit of quantitation; human; priority journal; article; gel permeation chromatography; mass spectrometer; ion cyclotron resonance mass spectrometry; time of flight mass spectrometry |
| Journal Title: | Advances in Experimental Medicine and Biology |
| Volume: | 806 |
| ISSN: | 0065-2598 |
| Publisher: | Springer |
| Date Published: | 2014-01-01 |
| Start Page: | 1 |
| End Page: | 32 |
| Language: | English |
| DOI: | 10.1007/978-3-319-06068-2_1 |
| PROVIDER: | scopus |
| PUBMED: | 24952176 |
| DOI/URL: | |
| Notes: | Chapter in "Advancements of Mass Spectrometry in Biomedical Research" (ISBN: 978-3-319-06067-5) -- Export Date: 2 September 2015 -- Source: Scopus |
