Automated multidimensional nanoscale chromatography for ultrasensitive targeted mass spectrometry Journal Article

   


Authors: Cifani, P.; Kentsis, A.
Article Title: Automated multidimensional nanoscale chromatography for ultrasensitive targeted mass spectrometry
Abstract: Recent advances in nanoscale separations and high-resolution mass spectrometry permit highly sensitive and accurate analyses of complex protein mixtures. Here, we describe improved methods for nanoscale multidimensional chromatography coupled to targeted mass spectrometry (tMS) to achieve ultrasensitive quantification of peptides in complex proteomes. The presented chromatographic system consists of capillary strong-cation exchange (SCX) chromatography column, from which peptides are eluted directly onto high-resolution reversed-phase (RP) analytical columns and nanoelectrospray ion source. SCX prefractionation is used to separate phosphorylated peptides, permitting their ultrasensitive quantification. Resolution and robustness of this chromatographic system, together with the orthogonality of SCX and RP separations, permit scheduling of large panels of targeted MS assays. This design also enables seamless scaling to three-dimensional separations, thereby enabling large-scale, ultrasensitive quantitative proteomics. © 2022, Springer Science+Business Media, LLC, part of Springer Nature.
Keywords: mass spectrometry; proteomics; chromatography; electrospray ionization; accumulated ion monitoring; multidimensional chromatography; nanoscale separation; reversed phase; strong cation exchange
Journal Title: Methods in Molecular Biology
Volume: 2393
ISSN: 1064-3745
Publisher: Humana Press Inc  
Date Published: 2022-01-01
Start Page: 207
End Page: 224
Language: English
DOI: 10.1007/978-1-0716-1803-5_11
PROVIDER: scopus
PUBMED: 34837181
PMCID: PMC10320743
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-85120645236&doi=10.1007%2f978-1-0716-1803-5_11&partnerID=40&md5=ca5bae9cb537ecf645a524bc32048545
Notes: Chapter 11 in "Biomedical Engineering Technologies" (ISBN: 978-1-0716-1802-8 -- Export Date: 3 January 2022 -- Source: Scopus
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MSK Authors
  1. Alex Kentsis
    103 Kentsis
  2. Paolo Cifani
    32 Cifani
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