Towards comprehensive and quantitative proteomics for diagnosis and therapy of human disease Journal Article


Authors: Cifani, P.; Kentsis, A.
Article Title: Towards comprehensive and quantitative proteomics for diagnosis and therapy of human disease
Abstract: Given superior analytical features, MS proteomics is well suited for the basic investigation and clinical diagnosis of human disease. Modern MS enables detailed functional characterization of the pathogenic biochemical processes, as achieved by accurate and comprehensive quantification of proteins and their regulatory chemical modifications. Here, we describe how high-accuracy MS in combination with high-resolution chromatographic separations can be leveraged to meet these analytical requirements in a mechanism-focused manner. We review the quantification methods capable of producing accurate measurements of protein abundance and posttranslational modification stoichiometries. We then discuss how experimental design and chromatographic resolution can be leveraged to achieve comprehensive functional characterization of biochemical processes in complex biological proteomes. Finally, we describe current approaches for quantitative analysis of a common functional protein modification: reversible phosphorylation. In all, current instrumentation and methods of high-resolution chromatography and MS proteomics are poised for immediate translation into improved diagnostic strategies for pediatric and adult diseases. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Keywords: mass spectrometry; functional proteomics; biomedicine; protein quantification; pediatric disease; ptm
Journal Title: Proteomics
Volume: 17
Issue: 1-2
ISSN: 1615-9853
Publisher: Wiley V C H Verlag Gmbh  
Date Published: 2017-01-01
Start Page: 1600079
Language: English
DOI: 10.1002/pmic.201600079
PROVIDER: scopus
PMCID: PMC5243792
PUBMED: 27775219
DOI/URL:
Notes: Review -- Export Date: 2 March 2017 -- Source: Scopus
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MSK Authors
  1. Alex   Kentsis
    50 Kentsis
  2. Paolo   Cifani
    17 Cifani