Analysis of meiotic recombination products from human sperm Journal Article
| Authors: | Kauppi, L.; May, C. A.; Jeffreys, A. J. |
| Article Title: | Analysis of meiotic recombination products from human sperm |
| Abstract: | Traditional methods for surveying meiotic recombination in humans are limited to pedigree and linkage disequilibrium analyses. We have developed assays that allow the direct detection of crossover and gene conversion molecules in batches of sperm DNA. To date, we have characterized 26 recombination hotspots by allele-specific PCR and selectively amplified recombinant DNA molecules from these regions. These analyses have revealed that meiotic crossover hotspots in humans are highly localized and flanked by DNA segments where recombination is suppressed. The centers of crossover hotspots are also active in noncrossover recombination, displaying short conversion tracts. |
| Keywords: | genetics; review; methodology; polymerase chain reaction; cytology; meiosis; metabolism; biological model; models, biological; algorithms; chemistry; genetic recombination; algorithm; recombination, genetic; chromosome analysis; cytogenetic analysis; spermatozoon; spermatozoa |
| Journal Title: | Methods in Molecular Biology |
| Volume: | 557 |
| ISSN: | 1064-3745 |
| Publisher: | Humana Press Inc |
| Date Published: | 2009-01-01 |
| Start Page: | 323 |
| End Page: | 355 |
| Language: | English |
| DOI: | 10.1007/978-1-59745-527-5_20 |
| PUBMED: | 19799191 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Chapter 20 in "Meiosis: Volume 1, Molecular and Genetic Methods" (ISBN: 978-1-934115-66-4) --- Cited By (since 1996): 2 - Export Date: 30 November 2010- Source: Scopus |
