PIKing the type and pattern of PI3K pathway mutations in endometrioid endometrial carcinomas Journal Article
| Authors: | Marchiò, C.; De Filippo, M. R.; Ng, C. K. Y.; Piscuoglio, S.; Soslow, R. A.; Reis-Filho, J. S.; Weigelt, B. |
| Article Title: | PIKing the type and pattern of PI3K pathway mutations in endometrioid endometrial carcinomas |
| Abstract: | Objective. The vastmajority of endometrioid endometrial carcinomas (EECs) harbor mutations in the PI3K pathway. Here we sought to determine whether the type and pattern ofmutations targeting different components of the PI3K pathway are distinct between microsatellite stable (MSS) and high-level microsatellite instable (MSI-H) EECs. Methods. Whole exome massively parallel sequencing-based mutation data from EECs of The Cancer Genome Atlas (TCGA) were used to define the number, type and pattern of mutations affecting PI3K pathway-related genes, including AKT1, INPP4B, MTOR, PIK3CA, PIK3R1 and PTEN. EECs were classified as MSI-H (n = 70) and MSS (n =109) based on seven MSI markers assessed by TCGA. Ultramutated cases were excluded. Results. Although themutation rates and mutational signatures of MSS and MSI-H EECs were distinct, the prevalence of PI3K pathway mutations was similar between these two groups (all p > 0.05), with the exception of PTEN mutations, which were more prevalent in MSI-H (61/70; 87%) than in MSS EECs (78/109; 72%; p = 0.017). The PIK3CA hotspot mutations E542K, E545K, and H1047R were found to be significantly more prevalent in PIK3CAmutant MSS (21/58, 36%) compared to PIK3CA-mutant MSI-H EECs (5/37, 13.5%; p=0.019). Conclusion. Although the prevalence of mutations targeting PI3K pathway genes is similar between MSS and MSI-H EECs, PIK3CA hotspot mutations, which result in constitutive kinase activation, are significantly more prevalent in MSS than in MSI-H EECs. Our findings warrant further investigation of the role of different types of PIK3CA mutations and their predictive impact on distinct subtypes of EECs. © 2015 Elsevier Inc. |
| Keywords: | signal transduction; protein kinase b; adult; cancer survival; event free survival; aged; unclassified drug; gene mutation; gene sequence; human cell; major clinical study; missense mutation; endometrioid carcinoma; endometrium carcinoma; protein domain; gene targeting; prevalence; phosphatidylinositol 3 kinase; cancer genetics; microsatellite instability; mammalian target of rapamycin; phosphatidylinositol 3,4,5 trisphosphate 3 phosphatase; mutation rate; nonsense mutation; mutations; pi3k pathway; indel mutation; endometrioid endometrial carcinoma; exome; hotspot; phosphatidylinositol 4,5 bisphosphate 3 kinase; human; female; priority journal; article; inositol polyphosphate; inositol polyphosphate 4 phosphatase; microsatellite stable; population genetic parameters |
| Journal Title: | Gynecologic Oncology |
| Volume: | 137 |
| Issue: | 2 |
| ISSN: | 0090-8258 |
| Publisher: | Elsevier Inc. |
| Date Published: | 2015-05-01 |
| Start Page: | 321 |
| End Page: | 328 |
| Language: | English |
| DOI: | 10.1016/j.ygyno.2015.02.010 |
| PROVIDER: | scopus |
| PUBMED: | 25701704 |
| DOI/URL: | |
| Notes: | Export Date: 3 August 2015 -- Source: Scopus |
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793Soslow -
632Weigelt -
639Reis-Filho -
155Ng -
130Piscuoglio -
27Marchio -
28De Filippo
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