Molecular profiling and molecular classification of endometrioid ovarian carcinomas Journal Article

Authors: Cybulska, P.; Da Cruz Paula, A.; Tseng, J.; Leitao, M. M. Jr; Bashashati, A.; Huntsman, D. G.; Nazeran, T. M.; Aghajanian, C.; Abu-Rustum, N. R.; DeLair, D. F.; Shah, S. P.; Weigelt, B.
Article Title: Molecular profiling and molecular classification of endometrioid ovarian carcinomas
Abstract: Objective: Endometrioid ovarian carcinomas (EOCs) comprise 5–10% of all ovarian cancers and commonly co-occur with synchronous endometrioid endometrial cancer (EEC). We sought to examine the molecular characteristics of pure EOCs in patients without concomitant EEC. Methods: EOCs and matched normal samples were subjected to massively parallel sequencing targeting 341–468 cancer-related genes (n = 8) or whole-genome sequencing (n = 28). Mutational frequencies of EOCs were compared to those of high-grade serous ovarian cancers (HGSOCs; n = 224) and EECs (n = 186) from The Cancer Genome Atlas, and synchronous EOCs (n = 23). Results: EOCs were heterogeneous, frequently harboring KRAS, PIK3CA, PTEN, CTNNB1, ARID1A and TP53 mutations. EOCs were distinct from HGSOCs at the mutational level, less frequently harboring TP53 but more frequently displaying KRAS, PIK3CA, PIK3R1, PTEN and CTNNB1 mutations. Compared to synchronous EOCs and pure EECs, pure EOCs less frequently harbored PTEN, PIK3R1 and ARID1A mutations. Akin to EECs, EOCs could be stratified into the four molecular subtypes: 3% POLE (ultramutated), 19% MSI (hypermutated), 17% copy-number high (serous-like) and 61% copy-number low (endometrioid). In addition to microsatellite instability, a subset of EOCs harbored potentially targetable mutations, including AKT1 and ERBB2 hotspot mutations. EOCs of MSI (hypermutated) subtype uniformly displayed a good outcome. Conclusions: EOCs are heterogeneous at the genomic level and harbor targetable genetic alterations. Despite the similarities in the repertoire of somatic mutations between pure EOCs, synchronous EOCs and EECs, the frequencies of mutations affecting known driver genes differ. Further studies are required to define the impact of the molecular subtypes on the outcome and treatment of EOC patients. © 2019 Elsevier Inc.
Keywords: heterogeneity; massively parallel sequencing; somatic mutations; molecular subtypes; endometrioid ovarian cancer
Journal Title: Gynecologic Oncology
Volume: 154
Issue: 3
ISSN: 0090-8258
Publisher: Elsevier Inc.  
Date Published: 2019-09-01
Start Page: 516
End Page: 523
Language: English
DOI: 10.1016/j.ygyno.2019.07.012
PUBMED: 31340883
PROVIDER: scopus
PMCID: PMC6736779
Notes: Article -- Export Date: 1 October 2019 -- Source: Scopus
Citation Impact
MSK Authors
  1. Mario Leitao
    375 Leitao
  2. Deborah F DeLair
    100 DeLair
  3. Britta Weigelt
    366 Weigelt
  4. Jill   Tseng
    25 Tseng
  5. Sohrab Prakash Shah
    15 Shah