FENS-1 and DFCP1 are FYVE domain-containing proteins with distinct functions in the endosomal and Golgi compartments Journal Article
| Authors: | Ridley, S. H.; Ktistakis, N.; Davidson, K.; Anderson, K. E.; Manifava, M.; Ellson, C. D.; Lipp, P.; Bootman, M.; Coadwell, J.; Nazarian, A.; Erdjument-Bromage, H.; Tempst, P.; Cooper, M. A.; Thuring, J. W. J. F.; Lim, Z. Y.; Holmes, A. B.; Stephens, L. R.; Hawkins, P. T. |
| Article Title: | FENS-1 and DFCP1 are FYVE domain-containing proteins with distinct functions in the endosomal and Golgi compartments |
| Abstract: | FENS-1 and DFCP1 are recently discovered proteins containing one or two FYVE-domains respectively. We show that the FYVE domains in these proteins can bind PtdIns3P in vitro with high specificity over other phosphoinositides. Exogenously expressed FENS-1 localises to early endosomes: this localisation requires an intact FYVE domain and is sensitive to wortmannin inhibition. The isolated FYVE domain of FENS-1 also localises to endosomes. These results are consistent with current models of FYVE-domain function in this cellular compartment. By contrast, exogenously expressed DFCP1 displays a predominantly Golgi, endoplasmic reticulum (ER) and vesicular distribution with little or no overlap with FENS-1 or other endosomal markers. Overexpression of DFCP1 was found to cause dispersal of the Golgi compartment defined by giantin and gpp130-staining. Disruption of the FYVE domains of DFCP1 causes a shift to more condensed and compact Golgi structures and overexpression of this mutant was found to confer significant protection to the Golgi against brefeldin-induced dispersal. These properties of DFCP1 are surprising, and suggest FYVE domain-localisation and function may not be exclusively endosomal. |
| Keywords: | cells; binding; localization; network; finger; membrane; vesicle trafficking; endosomes; vesicles; 3-kinase; golgi; fyve domain; ptdins3p; phosphatidylinositol 3-phosphate; rab |
| Journal Title: | Journal of Cell Science |
| Volume: | 114 |
| Issue: | 22 |
| ISSN: | 0021-9533 |
| Publisher: | Company of Biologists |
| Date Published: | 2001-11-15 |
| Start Page: | 3991 |
| End Page: | 4000 |
| Language: | English |
| ACCESSION: | WOS:000172594200005 |
| PROVIDER: | wos |
| PUBMED: | 11739631 |
| Notes: | Article -- Source: Wos |
Citation Impact
MSK Authors
Related MSK Work