Multiplex polymerase chain reaction tests for detection of pathogens associated with gastroenteritis Journal Article
| Authors: | Zhang, H.; Morrison, S.; Tang, Y. W. |
| Article Title: | Multiplex polymerase chain reaction tests for detection of pathogens associated with gastroenteritis |
| Abstract: | A wide range of enteric pathogens can cause infectious gastroenteritis. Conventional diagnostic algorithms are time-consuming and often lack sensitivity and specificity. Advances in molecular technology have provided new clinical diagnostic tools. Multiplex polymerase chain reaction (PCR)-based testing has been used in gastroenterology diagnostics in recent years. This article presents a review of recent laboratory-developed multiplex PCR tests and current commercial multiplex gastrointestinal pathogen tests. It focuses on two commercial syndromic multiplex tests: Luminex xTAG Gastrointestinal Pathogen Panel and BioFire FilmArray gastrointestinal test. Multiplex PCR tests have shown superior sensitivity to conventional methods for detection of most pathogens. © 2015 Elsevier Inc. |
| Keywords: | review; nonhuman; united states; diagnostic accuracy; laboratory diagnosis; sensitivity and specificity; clinical practice; clinical assessment; food and drug administration; assay; diagnostic value; medical research; microbiological examination; microorganism; gastroenteritis; molecular diagnosis; diagnostic equipment; multiplex pcr; multiplex polymerase chain reaction; human; priority journal; laboratory-developed test; device approval |
| Journal Title: | Clinics in Laboratory Medicine |
| Volume: | 35 |
| Issue: | 2 |
| ISSN: | 0272-2712 |
| Publisher: | Elsevier Inc. |
| Date Published: | 2015-06-01 |
| Start Page: | 461 |
| End Page: | 486 |
| Language: | English |
| DOI: | 10.1016/j.cll.2015.02.006 |
| PROVIDER: | scopus |
| PUBMED: | 26004652 |
| PMCID: | PMC5002946 |
| DOI/URL: | |
| Notes: | Export Date: 2 July 2015 -- Source: Scopus |
