Total synthesis of glycosylated proteins Journal Article
| Authors: | Fernández-Tejada, A.; Brailsford, J.; Zhang, Q.; Shieh, J.H.; Moore, M. A.; Danishefsky, S. J.; Liu, L. |
| Article Title: | Total synthesis of glycosylated proteins |
| Abstract: | Glycoproteins are an important class of naturally occurring biomolecules which play a pivotal role in many biological processes. They are biosynthesized as complex mixtures of glycoforms through post-translational protein glycosylation. This fact, together with the challenges associated with producing them in homogeneous form, has hampered detailed structure-function studies of glycoproteins as well as their full exploitation as potential therapeutic agents. By contrast, chemical synthesis offers the unique opportunity to gain access to homogeneous glycoprotein samples for rigorous biological evaluation. Herein, we review recent methods for the assembly of complex glycopeptides and glycoproteins and present several examples from our laboratory towards the total chemical synthesis of clinically relevant glycosylated proteins that have enabled synthetic access to full-length homogeneous glycoproteins. © Springer International Publishing Switzerland 2014. |
| Keywords: | glycosylation; glycoproteins; total synthesis; glycopeptides; chemoselective ligation |
| Journal Title: | Topics in Current Chemistry |
| Volume: | 362 |
| ISSN: | 0340-1022 |
| Publisher: | Springer |
| Date Published: | 2015-01-01 |
| Start Page: | 1 |
| End Page: | 26 |
| Language: | English |
| DOI: | 10.1007/128_2014_622 |
| PROVIDER: | scopus |
| PUBMED: | 25805144 |
| PMCID: | PMC5079620 |
| DOI/URL: | |
| Notes: | Chapter in "Protein Ligation and Total Synthesis I" (ISBN: 978-3-319-19185-0) -- Export Date: 3 June 2015 -- Source: Scopus |
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