The effects of soy supplementation on gene expression in breast cancer: A randomized placebo-controlled study Journal Article
| Authors: | Shike, M.; Doane, A. S.; Russo, L.; Cabal, R.; Reis-Filho, J. S.; Gerald, W.; Cody, H.; Khanin, R.; Bromberg, J.; Norton, L. |
| Article Title: | The effects of soy supplementation on gene expression in breast cancer: A randomized placebo-controlled study |
| Abstract: | Methods: Women (n = 140) with early-stage breast cancer were randomly assigned to soy protein supplementation (n = 70) or placebo (n = 70) for 7 to 30 days, from diagnosis until surgery. Adherence was determined by plasma isoflavones: genistein and daidzein. Gene expression changes were evaluated by NanoString in pre- and posttreatment tumor tissue. Genome-wide expression analysis was performed on posttreatment tissue. Proliferation (Ki67) and apoptosis (Cas3) were assessed by immunohistochemistry. Results: Plasma isoflavones rose in the soy group (two-sided Wilcoxon rank-sum test, P <.001) and did not change in the placebo group. In paired analysis of pre- and posttreatment samples, 21 genes (out of 202) showed altered expression (two-sided Student's t-test, P <.05). Several genes including FANCC and UGT2A1 revealed different magnitude and direction of expression changes between the two groups (two-sided Student's t-test, P <.05). A high-genistein signature consisting of 126 differentially expressed genes was identified from microarray analysis of tumors. This signature was characterized by overexpression (>2-fold) of cell cycle transcripts, including those that promote cell proliferation, such as FGFR2, E2F5, BUB1, CCNB2, MYBL2, CDK1, and CDC20 (P <.01). Soy intake did not result in statistically significant changes in Ki67 or Cas3. Conclusions: Gene expression associated with soy intake and high plasma genistein defines a signature characterized by overexpression of FGFR2 and genes that drive cell cycle and proliferation pathways. These findings raise the concerns that in a subset of women soy could adversely affect gene expression in breast cancer. Background: There are conflicting reports on the impact of soy on breast carcinogenesis. This study examines the effects of soy supplementation on breast cancer-related genes and pathways. © The Author 2014. Published by Oxford University Press. All rights reserved. |
| Journal Title: | JNCI: Journal of the National Cancer Institute |
| Volume: | 106 |
| Issue: | 9 |
| ISSN: | 0027-8874 |
| Publisher: | Oxford University Press |
| Date Published: | 2014-09-09 |
| Start Page: | dju189 |
| Language: | English |
| DOI: | 10.1093/jnci/dju189 |
| PUBMED: | 25190728 |
| PROVIDER: | scopus |
| PMCID: | PMC4817128 |
| DOI/URL: | |
| Notes: | Export Date: 3 June 2015 -- Source: Scopus |
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