Comparison of anti-prostate-specific membrane antigen antibodies and other immunomarkers in metastatic prostate carcinoma Journal Article
| Authors: | Chang, S. S.; Reuter, V. E.; Heston, W. D. W.; Gaudin, P. B. |
| Article Title: | Comparison of anti-prostate-specific membrane antigen antibodies and other immunomarkers in metastatic prostate carcinoma |
| Abstract: | Objectives. To compare the immunohistochemical properties of the 7E11 anti-prostate-specific membrane antigen (anti-PSMA) monoclonal antibody (mAb) with the recently developed anti-PSMA mAb, PM2J004.5, and with other common immunomarkers in metastatic prostate cancer. PSMA is a type II integral membrane glycoprotein highly expressed in prostate cancer cells. The mAb 7E11 is currently used in the radioisotopic evaluation of prostate cancer, and its immunohistochemical properties have been examined in primary prostate cancer specimens. Methods. We examined 23 formalin-fixed, paraffin-embedded, metastatic prostate carcinoma specimens from various anatomic sites, including bone, lymph node, liver, lung, and soft tissue. Using the biotin-streptavidin method, we performed immunohistochemical reactions with the anti-PSMA mAbs 7E11 and PM2J004.5 and with antibodies to prostate-specific antigen and prostatic acid phosphatase. The immunoreactions were scored by pathologists unaware of the clinical and pathologic data according to a staining intensity scale and the percentage of cells stained. Results. All four mAbs consistently stained the metastatic prostate cancer specimens. In 2 (8.7%) of 23 cases, however, the prostate-specific antigen immunoreaction was negative but the anti-PSMA mAbs had positive staining. Although 7E11 and PM2J004.5 had a similar staining intensity and percentage of cells stained for most specimens, in 3 (13%) of 23 specimens, 7E11 had less intense staining. None of the specimens were negative for all four antibodies. Conclusions. Anti-PSMA mAbs consistently immunoreacted with metastatic prostate cancer specimens and were positive in instances when prostate-specific antigen staining was negative. The anti-PSMA mAbs demonstrated similar staining patterns; however, in select cases, the PM2J004.5 mAb did show more intense staining. The anti-PSMA mAbs 7E11 and PM2J004.5 are useful in the pathologic evaluation of paraffin-embedded metastatic prostate cancer specimens. © 2001 Elsevier Science Inc. |
| Keywords: | immunohistochemistry; controlled study; human tissue; unclassified drug; cancer localization; comparative study; antigen expression; biological marker; prostate specific antigen; metastasis; tumor markers, biological; immunoreactivity; monoclonal antibody; prostatic neoplasms; prostate specific membrane antigen; antibodies, monoclonal; diagnostic agent; cancer cell; membrane protein; radiodiagnosis; antibody; streptavidin; formaldehyde; glutamate carboxypeptidase ii; antigens, surface; prostate carcinoma; paraffin; biotin; paraffin embedding; acid phosphatase prostate isoenzyme; humans; human; male; priority journal; article; monoclonal antibody 7e11; carboxypeptidases; monoclonal antibody pm2j004 5 |
| Journal Title: | Urology |
| Volume: | 57 |
| Issue: | 6 |
| ISSN: | 0090-4295 |
| Publisher: | Elsevier Science, Inc. |
| Date Published: | 2001-06-01 |
| Start Page: | 1179 |
| End Page: | 1183 |
| Language: | English |
| DOI: | 10.1016/s0090-4295(01)00983-9 |
| PUBMED: | 11377343 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Export Date: 21 May 2015 -- Source: Scopus |
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