Authors: | Gilewski, T.; Ragupathi, G.; Bhuta, S.; Williams, L. J.; Musselli, C.; Zhang, X. F.; Bencsath, K. P.; Panageas, K. S.; Chin, J.; Hudis, C. A.; Norton, L.; Houghton, A. N.; Livingston, P. O.; Danishefsky, S. J. |
Article Title: | Immunization of metastatic breast cancer patients with a fully synthetic globo H conjugate: A phase I trial |
Abstract: | The carbohydrate antigen globo H commonly found on breast cancer cells is a potential target for vaccine therapy. The objectives of this trial were to determine the toxicity and immunogenicity of three synthetic globo H-keyhole limpet hemocyanin conjugates plus the immunologic adjuvant QS-21. Twenty-seven metastatic breast cancer patients received five vaccinations each. The vaccine was well tolerated, and no definite differences were observed among the three formulations. Serologic analyses demonstrated the generation of IgM antibody titers in most patients, with minimal IgG antibody stimulation. There was significant binding of IgM antibodies to MCF-7 tumor cells in 16 patients, whereas IgG antibody reactivity was observed in a few patients. There was evidence of complement-dependent cytotoxicity in several patients. Affinity column purification supported the specificity of IgM antibodies for globo H. On the basis of these data, globo H will constitute one component of a polyvalent vaccine for evaluation in high-risk breast cancer patients. |
Keywords: | adult; clinical article; controlled study; treatment outcome; bone neoplasms; middle aged; unclassified drug; human cell; clinical trial; drug tolerability; fatigue; liver neoplasms; lymphatic metastasis; metastasis; controlled clinical trial; breast cancer; randomized controlled trial; lung neoplasms; myalgia; breast neoplasms; enzyme linked immunosorbent assay; fever; pruritus; rigor; molecular sequence data; cancer vaccine; cancer vaccines; antibody response; cancer immunization; neoplasm metastasis; headache; phase 1 clinical trial; immunoglobulin g antibody; drug conjugation; carbohydrate sequence; carbohydrate antigen; antibody titer; drug formulation; complement dependent cytotoxicity; keyhole limpet hemocyanin; immunoglobulin m antibody; vaccines, conjugate; immunization; globo h antigen; qs 21; cancer antibody; humans; human; female; priority journal; article; mink cell focus-forming virus |
Journal Title: | Proceedings of the National Academy of Sciences of the United States of America |
Volume: | 98 |
Issue: | 6 |
ISSN: | 0027-8424 |
Publisher: | National Academy of Sciences |
Date Published: | 2001-03-13 |
Start Page: | 3270 |
End Page: | 3275 |
Language: | English |
DOI: | 10.1073/pnas.051626298 |
PUBMED: | 11248068 |
PROVIDER: | scopus |
PMCID: | PMC30643 |
DOI/URL: | |
Notes: | Export Date: 21 May 2015 -- Source: Scopus |