Downregulation of the retinoblastoma gene expression in the progression of malignant melanoma Journal Article


Authors: Korabiowska, M.; Ruschenburg, I.; Betke, H.; Stachura, J.; Schlott, T.; Cardo, C. C.; Brinck, U.
Article Title: Downregulation of the retinoblastoma gene expression in the progression of malignant melanoma
Abstract: The retinoblastoma gene is a cell cycle regulator preventing cells from entering into S-phase. An altered expression of the retinoblastoma gene has been reported in the majority of human malignancies. The main aim of this study was to investigate retinoblastoma gene expression in the full spectrum of melanoma progression from naevus to melanoma metastases by applying immunohistochemistry and RT-PCR. All naevi with and without dysplasia showed high expression of the retinoblastoma gene. In primary melanomas, Rb-positive cells were found in 82 out of 106. Loss of expression correlated with an increase in Clark level and shorter survival rates. An independent prognostic role of the retinoblastoma gene was confirmed by Cox multivariate analyses (p < 0.01). In melanoma metastases, retinoblastoma gene expression (at the RNA level) was found in 18 out of 26 melanoma lymphatic metastases, and in 2 out of 5 liver metastases. Our results indicate a downregulation of the retinoblastoma gene in the progression of melanocytic tumours. Copyright © 2002 S. Karger AG, Basel.
Keywords: immunohistochemistry; adolescent; adult; cancer survival; child; controlled study; human tissue; aged; aged, 80 and over; middle aged; survival rate; human cell; major clinical study; cancer growth; lymph node metastasis; lymph nodes; lymphatic metastasis; neoplasm staging; genetic analysis; melanoma; metastasis; reverse transcription polymerase chain reaction; gene expression; nevus; skin neoplasms; down-regulation; retinoblastoma; cell assay; rna; liver metastasis; correlation analysis; reverse transcriptase polymerase chain reaction; disease progression; down regulation; multivariate analysis; metastases; retinoblastoma protein; rna, neoplasm; retinoblastoma gene; genes, retinoblastoma; humans; prognosis; human; male; female; priority journal; article; rna expression
Journal Title: Pathobiology
Volume: 69
Issue: 5
ISSN: 1015-2008
Publisher: S. Karger AG  
Date Published: 2001-06-01
Start Page: 274
End Page: 280
Language: English
DOI: 10.1159/000064338
PUBMED: 12107345
PROVIDER: scopus
DOI/URL:
Notes: Export Date: 21 May 2015 -- Source: Scopus
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