Timely release of both replication forks from oriC requires modulation of origin topology Journal Article
| Authors: | Smelkova, N.; Marians, K. J. |
| Article Title: | Timely release of both replication forks from oriC requires modulation of origin topology |
| Abstract: | Initiation of DNA replication at oriC occurs bidirectionally both in vivo and in vitro. Although the proteins involved in establishing the replication forks are known, little is known about the events that ensure that initiation is bidirectional. We show here that in the absence of DNA gyrase, replication fork progression from oriC on a plasmid template in vitro is unidirectional, although both replication forks have formed at the origin. There was no bias in the release of one fork or the other, ruling out protein blockage of one fork as a possible reason for the asymmetric release. Timely release of both forks required the presence of either DNA gyrase or topoisomerase IV, suggesting that modulation of the topology of the origin region is the governing factor. |
| Keywords: | unclassified drug; genetics; dna replication; protein localization; metabolism; biological model; protein binding; gene product; enzymology; physiology; time; time factors; blotting, western; bacterial protein; bacterial proteins; dna; kinetics; escherichia coli; western blotting; plasmid; models, genetic; helicase; dna topoisomerase (atp hydrolysing); biochemistry; enzymes; dna helicases; dna topoisomerase iv; replication fork; gene replication; topology; dna template; cross linking reagent; cross-linking reagents; dnab helicases; priority journal; article; dna gyrase; protein oric; dnab helicase |
| Journal Title: | Journal of Biological Chemistry |
| Volume: | 276 |
| Issue: | 42 |
| ISSN: | 0021-9258 |
| Publisher: | American Society for Biochemistry and Molecular Biology |
| Date Published: | 2001-10-19 |
| Start Page: | 39186 |
| End Page: | 39191 |
| Language: | English |
| DOI: | 10.1074/jbc.M104411200 |
| PUBMED: | 11504719 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Export Date: 21 May 2015 -- Source: Scopus |
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