Topoisomerase III, but not topoisomerase I, can support nascent chain elongation during theta-type DNA replication Journal Article
| Authors: | Hiasa, H.; Marians, K. J. |
| Article Title: | Topoisomerase III, but not topoisomerase I, can support nascent chain elongation during theta-type DNA replication |
| Abstract: | Topoisomerase III, but not topoisomerase I, could, in the absence of DNA gyrase, support bidirectional DNA replication in an oriC plasmid DNA replication system reconstituted with purified proteins. The initial rate of DNA synthesis and the efficiency of nascent chain elongation showed that topoisomerase III-stimulated DNA replication was as efficient as gyrase- stimulated DNA replication. In addition, topoisomerase III was also able, in the absence of DNA gyrase, to decatenate the replicating daughter DNA molecules to form monomer product. Thus, of the four topoisomerases in Escherichia coli, three, topoisomerases III and IV and DNA gyrase, can support nascent chain elongation, whereas only two, topoisomerases III and IV, can topologically resolve the daughter molecules. |
| Keywords: | nonhuman; dna replication; dna synthesis; escherichia coli; gene insertion; dna topoisomerase (atp hydrolysing); polydeoxyribonucleotide synthase; dna topoisomerases, type ii; dna topoisomerase; dna topoisomerases, type i; plasmid dna; gel electrophoresis; ribonuclease h; bacterial enzyme; replication origin; priority journal; article; support, u.s. gov't, p.h.s.; recombinant plasmid |
| Journal Title: | Journal of Biological Chemistry |
| Volume: | 269 |
| Issue: | 51 |
| ISSN: | 0021-9258 |
| Publisher: | American Society for Biochemistry and Molecular Biology |
| Date Published: | 1994-12-23 |
| Start Page: | 32655 |
| End Page: | 32659 |
| Language: | English |
| PROVIDER: | scopus |
| PUBMED: | 7798272 |
| DOI/URL: | |
| Notes: | Export Date: 14 January 2019 -- Article -- Source: Scopus |
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