Molecular cloning of the CA125 ovarian cancer antigen: Identification as a new mucin, MUC16 Journal Article
| Authors: | Yin, B. W. T.; Lloyd, K. O. |
| Article Title: | Molecular cloning of the CA125 ovarian cancer antigen: Identification as a new mucin, MUC16 |
| Abstract: | CA125 is an ovarian cancer antigen that is the basis for a widely used serum assay for the monitoring of patients with ovarian cancer; however, detailed information on its biochemical and molecular nature is lacking. We now report the isolation of a long, but partial, cDNA that corresponds to the CA125 antigen. A rabbit polyclonal antibody produced to purified CA125 antigen was used to screen a λZAP cDNA library from OVCAR-3 cells in Escherichia coli. The longest insert from the 54 positive isolated clones had a 5797-base pair sequence containing a stop codon and a poly(A) sequence but no clear 5′ initiation sequence. The deduced amino acid sequence has many of the attributes of a mucin molecule and was designated CA125/MUC16 (gene MUC16). These features include a high serine, threonine, and proline content in an N-terminal region of nine partially conserved tandem repeats (156 amino acids each) and a C-terminal region non-tandem repeat sequence containing a possible transmembrane region and a potential tyrosine phosphorylation site. Northern blotting showed that the level of MUC16 mRNA correlated with the expression of CA125 in a panel of cell lines. The molecular cloning of the CA125 antigen will lead to a better understanding of its role in ovarian cancer. |
| Keywords: | protein phosphorylation; unclassified drug; human cell; genetics; stop codon; molecular genetics; antigen expression; ovarian neoplasms; proteins; chromosome 19; ovary cancer; serine; carboxy terminal sequence; transcription initiation; patient monitoring; tumor cells, cultured; molecular cloning; cloning, molecular; immunology; amino acid sequence; molecular sequence data; sequence homology, amino acid; amino terminal sequence; antigens; cell culture; messenger rna; tumors; 5' untranslated region; nucleotide sequence; ovary tumor; escherichia coli; base sequence; chromosomes, human, pair 19; amino acid; base pairing; threonine; antibodies; ca 125 antigen; ca-125 antigen; amino acids; codon; sequence homology; patient treatment; biochemistry; blotting, northern; complementary dna; assays; chromosome map; chromosome mapping; proline; dna, complementary; northern blotting; mucin; mucins; cloning; tandem repeat; polyadenylic acid; polyclonal antibody; dna library; oryctolagus cuniculus; mucin 16; humans; human; female; priority journal; article |
| Journal Title: | Journal of Biological Chemistry |
| Volume: | 276 |
| Issue: | 29 |
| ISSN: | 0021-9258 |
| Publisher: | American Society for Biochemistry and Molecular Biology |
| Date Published: | 2001-07-20 |
| Start Page: | 27371 |
| End Page: | 27375 |
| Language: | English |
| DOI: | 10.1074/jbc.M103554200 |
| PUBMED: | 11369781 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Export Date: 21 May 2015 -- Source: Scopus |
Altmetric
Citation Impact
BMJ Impact Analytics
Related MSK Work