Ovarian cancer antigen CA125 is encoded by the MUC16 mucin gene Journal Article
| Authors: | Yin, B. W. T.; Dnistrian, A.; Lloyd, K. O. |
| Article Title: | Ovarian cancer antigen CA125 is encoded by the MUC16 mucin gene |
| Abstract: | Serum assays based on the CA125 antigen are widely used in the monitoring of patients with ovarian cancer; however very little is known about the molecular nature of the CA125 antigen. We recently cloned a partial cDNA (designated MUC16) that codes for a new mucin that is a strong candidate for being the CA125 antigen. This assignment has now been confirmed by transfecting a partial MUC16 cDNA into 2 CA125-negative cell lines and demonstrating the synthesis of CA125 by 3 different assays. Of the 3 antibodies (OC125, M1 1 and VK-8) tested on the transfected cells, only the first 2 were strongly positive, indicating the differential expression of the CA125 epitopes in these cells. The cloning and expression of CA125 antigen opens the way to an understanding of its function in normal and malignant cells. © 2002 Wiley-Liss, Inc. |
| Keywords: | controlled study; human cell; ovarian cancer; antigen expression; ovarian neoplasms; ovary cancer; gene expression; membrane proteins; immunofluorescence; tumor cells, cultured; transfection; monoclonal antibody; molecular cloning; fluorescent antibody technique; amino acid sequence; molecular sequence data; nucleotide sequence; epitope; ca 125 antigen; ca-125 antigen; serum; complementary dna; dna, complementary; mucin; epitopes; muc16; radioimmunoprecipitation; humans; human; female; priority journal; article; ca125 antigen; ca125 epitopes; radioimmunoprecipitation assay |
| Journal Title: | International Journal of Cancer |
| Volume: | 98 |
| Issue: | 5 |
| ISSN: | 0020-7136 |
| Publisher: | John Wiley & Sons |
| Date Published: | 2002-04-10 |
| Start Page: | 737 |
| End Page: | 740 |
| Language: | English |
| DOI: | 10.1002/ijc.10250 |
| PUBMED: | 11920644 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Export Date: 14 November 2014 -- Source: Scopus |
Altmetric
Citation Impact
BMJ Impact Analytics
Related MSK Work