The use of in ovo electroporation for the rapid analysis of neural-specific murine enhancers Journal Article
| Authors: | Timmer, J.; Johnson, J.; Niswander, L. |
| Article Title: | The use of in ovo electroporation for the rapid analysis of neural-specific murine enhancers |
| Abstract: | The identification and characterization of DNA sequences necessary for proper gene expression have provided insights into gene regulation and generated tools useful for further experimentation. Studies of developmentally regulated genes have demonstrated how transcription factors interact at enhancers to generate restricted patterns of expression during embryogenesis. In vertebrates, the pursuit of such studies has relied on the generation of transgenic mice and thus has been limited by the time and expense required generating and characterizing these mice. The recently developed technique of in ovo electroporation allows the rapid introduction of exogenous DNA into developing chicken embryos. Here we have used this technique to introduce DNA containing murine enhancer/reporter constructs into cells of the chicken neural tube, resulting in appropriate expression of the reporter. This technique has the potential to greatly reduce the effort involved in the study of vertebrate enhancers. Furthermore, we have characterized factors such as timing of electroporation, concentration of DNA, and choice of basal promoters and found that they can influence the degree to which expression of enhancer constructs reflects endogenous gene expression. © 2001 Wiley-Liss, Inc. |
| Keywords: | promoter region; nonhuman; mouse; animals; mice; gene expression profiling; embryo; analytic method; basic helix-loop-helix transcription factors; nerve tissue proteins; transcription factor; embryo development; neurons; time factors; transgenic mouse; mus musculus; transcription factors; vertebrata; gene expression regulation; murinae; reporter gene; electroporation; dna sequence; chick embryo; enhancer region; chicken; neural; enhancer elements (genetics); promoter regions (genetics); bhlh; priority journal; article; gallus gallus; enhancer analysis |
| Journal Title: | Genesis |
| Volume: | 29 |
| Issue: | 3 |
| ISSN: | 1526-954X |
| Publisher: | Wiley Blackwell |
| Date Published: | 2001-03-01 |
| Start Page: | 123 |
| End Page: | 132 |
| Language: | English |
| DOI: | 10.1002/gene.1015 |
| PUBMED: | 11252053 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Export Date: 21 May 2015 -- Source: Scopus |
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