Absence of post-transcriptional RNA modifications of BCL10 in human malignant mesothelioma and colorectal cancer Journal Article
| Authors: | Apostolou, S.; Murthy, S. S.; Kolachana, P.; Jhanwar, S. C.; Testa, J. R. |
| Article Title: | Absence of post-transcriptional RNA modifications of BCL10 in human malignant mesothelioma and colorectal cancer |
| Abstract: | The BCL10 gene, located at 1p22, has been implicated in a number of human malignancies, including malignant mesotheliomas (MMs) and colorectal carcinomas. Subsequent reports, however, have revealed an absence of BCL10 mutations in genomic DNA from such tumors. It has been proposed that some abnormalities of this gene may be found only in RNA and not in genomic DNA, suggesting that BCL10 may be mutated post-transcriptionally, rather than at the genomic level. To explore this possibility, we performed SSCP mutation analysis and direct sequencing of cDNA from 17 MM cell lines displaying LOH in 1p22, 12 MM tumor specimens, and 11 colon carcinoma cell lines. SSCP revealed several different band shifts in these samples. The nucleotide changes observed in the cDNA samples were also seen in matched genomic DNA and corresponded to known polymorphisms in the general population. Thus, we conclude the BCL10 mutations are absent at the cDNA level, and that this gene does not undergo 'molecular misreading.' Since BCL10 also does not possess mutations at the genomic DNA level, it can be ruled out as a gene involved in the pathogenesis of MM and colorectal cancer. (C) 2001 Wiley-Liss, Inc. |
| Keywords: | gene mutation; human cell; mutation; sequence deletion; colorectal cancer; proto oncogene; neoplasm proteins; genetic transcription; cancer cell culture; tumor cells, cultured; carcinogenesis; colorectal neoplasms; rna; malignant mesothelioma; mesothelioma; adaptor proteins, signal transducing; genome; dna sequence; dna mutational analysis; chromosome aberrations; point mutation; rna, neoplasm; gene location; complementary dna; rna processing, post-transcriptional; dna polymorphism; single strand conformation polymorphism; polymorphism, single-stranded conformational; chromosome disorders; humans; human; priority journal; article |
| Journal Title: | Genes Chromosomes and Cancer |
| Volume: | 30 |
| Issue: | 1 |
| ISSN: | 1045-2257 |
| Publisher: | Wiley Periodicals, Inc |
| Date Published: | 2001-01-01 |
| Start Page: | 96 |
| End Page: | 98 |
| Language: | English |
| DOI: | 10.1002/1098-2264(2000)9999:9999<::aid-gcc1059>3.0.co;2-7 |
| PUBMED: | 11107182 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Export Date: 21 May 2015 -- Source: Scopus |
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