Mutational study of p16(CDKN2/MTS1/INK4A) and p57(KIP2) genes in hepatocellular carcinoma Journal Article

  


Authors: Bonilla, F.; Orlow, I.; Cordon-Cardo, C.
Article Title: Mutational study of p16(CDKN2/MTS1/INK4A) and p57(KIP2) genes in hepatocellular carcinoma
Abstract: p16 and p15 are representative members of cyclin-dependent kinase inhibitors. Because the selective expression of p57(KIP2) in liver, and because p16(CDKN2/MTS1/INK4A) has been found altered in many primary tumors, we undertook the present study to determine the presence of alterations in these genes in a group of hepatocellular carcinomas (HCC). Seventeen tumor and normal DNA pairs were analyzed by Southern blot, PCR-SSCP and DNA sequencing. Microsatellite markers surrounding the area of the p16 gene was also used. Southern blot analysis did not show allelic losses of the p16 or p57(KIP2) genes. In 4 cases, an extra band was observed when hybridizing with the specific p16 cDNA. Overall, 4/17 (24%) cases presented microsatellite alterations at the 9p21-24 region. These results suggest that deletions or point mutations in these genes are not frequent if present at all in HCC, but reveals the existence of microsatellite alterations at the 9p21-24 region in HCC.
Keywords: hepatocellular carcinoma; dna; carcinoma; p16; squamous-cell; dependent kinase inhibitor; tumor-suppressor gene; deletions; cycle; deletion; cancers; human; human bladder-tumors; p15 genes; p57; microsatellite alterations
Journal Title: International Journal of Oncology
Volume: 12
Issue: 3
ISSN: 1019-6439
Publisher: Spandidos Publications  
Date Published: 1998-03-01
Start Page: 583
End Page: 588
Language: English
ACCESSION: WOS:000072229900014
PROVIDER: wos
PUBMED: 9472096
DOI: 10.3892/ijo.12.3.583
Notes: Article -- Source: Wos
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MSK Authors
  1. Carlos Cordon-Cardo
    612 Cordon-Cardo
  2. Irene Orlow
    247 Orlow
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