Lack of correlation of functional scintigraphy with 99mtechnetium-methoxyisobutylisonitrile with histological necrosis following induction chemotherapy or measures of P-glycoprotein expression in high-grade osteosarcoma Journal Article
| Authors: | Gorlick, R.; Liao, A. C.; Antonescu, C.; Huvos, A. G.; Healey, J. H.; Sowers, R.; Daras, M.; Calleja, E.; Wexler, L. H.; Panicek, D.; Meyers, P. A.; Yeh, S. D.; Larson, S. M. |
| Article Title: | Lack of correlation of functional scintigraphy with 99mtechnetium-methoxyisobutylisonitrile with histological necrosis following induction chemotherapy or measures of P-glycoprotein expression in high-grade osteosarcoma |
| Abstract: | In osteosarcoma, some studies have suggested P-glycoprotein expression is a prognostic factor. The clearance of 99mtechnetium hexakis-2-methoxyisobutylisonitrile (99mTc-MIBI) has been used in some tumor systems as an in vivo measure of P-glycoprotein-mediated efflux. In this study we explored the correlation between 99mTc-MIBI clearance and histological necrosis following induction chemotherapy and P-glycoprotein expression in osteosarcoma. The primary tumors of 20 patients with high-grade osteosarcoma were imaged at diagnosis with 99mTc-MIBI, and the uptake ratios and biological half-lives were calculated. P-Glycoprotein expression in the tumor tissue was determined immunohistochemically and by measuring mRNA expression of the multidrug resistance-1 gene. The histological necrosis following induction chemotherapy was assessed by the Huvos grading system. The biological half-life of 99mTc-MIBI ranged from 1.4 to 52.5 h. Seven of the 20 tumor samples had a favorable extent of necrosis following induction chemotherapy. The 99mTc-MIBI half-life and uptake ratio showed no correlation with histological necrosis following induction chemotherapy. The 99mTc-MIBI half-life and uptake ratio did not correlate with either measure of P-glycoprotein expression. The results of this pilot study indicate that 99mTc-MIBI imaging is not an effective predictor of histological necrosis following induction chemotherapy in high-grade osteosarcoma. 99mTc-MIBI imaging did not correlate with measures of P-glycoprotein expression in the tumor tissue. |
| Keywords: | immunohistochemistry; osteosarcoma; adolescent; adult; cancer chemotherapy; child; clinical article; controlled study; human tissue; protein expression; school child; bone neoplasms; histopathology; cisplatin; doxorubicin; methotrexate; image analysis; necrosis; prediction; gene expression regulation, neoplastic; diagnostic value; reverse transcriptase polymerase chain reaction; pilot projects; folinic acid; methoxy isobutyl isonitrile technetium tc 99m; bone and bones; drug half life; rna, neoplasm; p-glycoprotein; technetium tc 99m sestamibi; glycoprotein p; correlation function; humans; prognosis; human; male; female; priority journal; article |
| Journal Title: | Clinical Cancer Research |
| Volume: | 7 |
| Issue: | 10 |
| ISSN: | 1078-0432 |
| Publisher: | American Association for Cancer Research |
| Date Published: | 2001-10-01 |
| Start Page: | 3065 |
| End Page: | 3070 |
| Language: | English |
| PUBMED: | 11595696 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Export Date: 21 May 2015 -- Source: Scopus |
