A phase I toxicity, pharmacology, and dosimetry trial of monoclonal antibody OKB7 in patients with non-Hodgkin's lymphoma: Effects of tumor burden and antigen expression Journal Article

  

Authors:	Scheinberg, D. A.; Straus, D. J.; Yeh, S. D.; Divgi, C.; Garin-Chesa, P.; Graham, M.; Pentlow, K.; Coit, D.; Oettgen, H. F.; Old, L. J.
Article Title:	A phase I toxicity, pharmacology, and dosimetry trial of monoclonal antibody OKB7 in patients with non-Hodgkin's lymphoma: Effects of tumor burden and antigen expression
Abstract:	Eighteen patients with relapsed non-Hodgkin's lymphoma (NHL) were infused with escalating doses of monoclonal antibody (mAb) OKB7, trace-labeled with iodine-131 (131l), in order to study toxicity, pharmacology, antibody localization, and dosimetry of radioiodine. OKB7 is a noncytotoxic mouse immunoglobulin G2b (lgG2b) mAb reactive with B cells and most B-cell NHL. Three patients each were treated at six dose levels ranging from 0.1 mg to 40 mg. All patients had radionuclide imaging and counting daily, had serial blood sampling to study pharmacokinetics, human antimouse antibody (HAMA), and circulating antigen, and had a biopsy of accessible lymphoma to determine delivery of isotope to tumors and assess the effect of tumor antigen expression on mAb delivery. Bone marrow biopsies were also done in the majority of patients. There was no toxicity. Serum clearance showed a median early phase half-life of 1.9 hours and a later phase half-life of 21.7 hours. Median total body clearance half-life was 22 hours. Pharmacokinet-ics were not dose-related. HAMA was detected in five patients. Circulating blocking antigen was detected in the serum of four patients, but at levels that were of pharmacologic consequence only in one. Biopsied tumor tissue from five patients did not express OKB7 antigen. No significant uptake of antibody was seen in these tumor sites. Mean total uptake of isotope into lymphoma measured in biopsies correlated linearly over the 400-fold increase in injected mAb dose. However, the percent of injected dose found per gram of tumor was unrelated to dose, but correlated inversely with tumor burden. In two patients with minimal tumor burden, 1.0 mg and 5.0 mg doses of OKB7 resulted in tumor to body radioisotope dose ratios of 22 and 7, which would theoretically permit tolerable delivery of 4,400 and 1,400 rods to these tumors, respectively, if OKB7 were conjugated with higher doses of 131l. © 1990 by American Society of Clinical Oncology.
Keywords:	adult; clinical article; aged; unclassified drug; animal; mice; age; b-lymphocytes; monoclonal antibody; antibodies, monoclonal; nonhodgkin lymphoma; antigens, neoplasm; tissue distribution; iodine radioisotopes; lymphoma, non-hodgkin; metabolic clearance rate; radiometry; phase 1 clinical trial; radioisotope; half-life; middle age; drug evaluation; radioiodination; human; male; female; priority journal; article; support, non-u.s. gov't; monoclonal antibody okb7
Journal Title:	Journal of Clinical Oncology
Volume:	8
Issue:	5
ISSN:	0732-183X
Publisher:	American Society of Clinical Oncology
Date Published:	1990-05-01
Start Page:	792
End Page:	803
Language:	English
DOI:	10.1200/jco.1990.8.5.792
PUBMED:	2332769
PROVIDER:	scopus
DOI/URL:	https://www.scopus.com/inward/record.uri?eid=2-s2.0-0025216138&doi=10.1200%2fJCO.1990.8.5.792&partnerID=40&md5=4c2c99ead071090b662f48e47e14dadd
Notes:	Source: Scopus

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