Cdk pathway: Cyclin-dependent kinases and cyclin-dependent kinase inhibitors Journal Article
| Authors: | Gitig, D.M.; Koff, A. |
| Article Title: | Cdk pathway: Cyclin-dependent kinases and cyclin-dependent kinase inhibitors |
| Abstract: | Many mechanisms either activate or inhibit the cdks and thereby either promote or arrest progression through the mitotic cell cycle. Since the signal transduction pathways emanating from extracellular mitogens and the agents controlling these pathways are complicated there may yet be novel mechanisms of cell cycle regulation remaining to be elucidated. In this article we outline the different techniques used to study the cell cycle and its regulation. These include: establishing that the cell cycle is arrested by propidium iodide staining followed by FACS analysis or by measuring 3H-thymidine incorporation into DNA; measuring the amount of cyclin/cdk associated kinase activity; assessing the steady-state expression profiles of cyclins, cdks and ckis by immunoblotting; and investigating the formation of complexes between these proteins by coimmunoprecipitations. Caveats and advantages of each technique are discussed. Following this paradigm yielded the discovery of the cell cycle inhibitors p27Kip1 and p21Cip1 and could very well lead to the discovery or novel cell cycle regulatory mechanisms. |
| Keywords: | signal transduction; protein expression; flow cytometry; animals; cell cycle proteins; mice; cell cycle; complex formation; models, biological; steady state; protein kinases; enzyme activity; phosphorylation; blotting, western; dna; regulatory mechanism; enzyme inhibitors; protein p27; cyclin-dependent kinase inhibitor p27; tumor suppressor proteins; western blotting; glutathione transferase; immunoblotting; cyclin-dependent kinase inhibitor p21; cycline; cyclin-dependent kinases; cyclins; cyclin dependent kinase; cyclin dependent kinase inhibitor; retinoblastoma protein; protein p21; cell separation; thymidine; cyclin; coloring agents; kinase assay; precipitin tests; article; propidium; cdk (cyclin dependent kinase); cki (cyclin dependent kinase inhibitor); coimmunoprecipitation; facs(fluorescent activated cell sorting); immunoblot (western blot) |
| Journal Title: | Molecular Biotechnology |
| Volume: | 19 |
| Issue: | 2 |
| ISSN: | 1073-6085 |
| Publisher: | Humana Press Inc |
| Date Published: | 2001-10-01 |
| Start Page: | 179 |
| End Page: | 188 |
| Language: | English |
| DOI: | 10.1385/mb:19:2:179 |
| PUBMED: | 11725487 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Export Date: 21 May 2015 -- Source: Scopus |
