A cyclin-dependent kinase-activating kinase (CAK) in budding yeast unrelated to vertebrate CAK Journal Article
| Authors: | Espinoza, F. H.; Farrell, A.; Erdjument-Bromage, H.; Tempst, P.; Morgan, D. O. |
| Article Title: | A cyclin-dependent kinase-activating kinase (CAK) in budding yeast unrelated to vertebrate CAK |
| Abstract: | Progress through the cell cycle is governed by the cyclin-dependent kinases (CDKs), the activation of which requires phosphorylation by the CDK- activating kinase (CAK). In vertebrates, CAK is a trimeric enzyme containing CDK7, cyclin H, and MAT1. CAK from the budding yeast Saccharomyces cerevisiae was identified as an unusual 44-kilodalton protein kinase, Cak1, that is only distantly related to CDKs. Cak1 accounted for most CAK activity in yeast cell lysates, and its activity was constant throughout the cell cycle. The CAK1 gene was essential for cell viability. Thus, the major CAK in S. cerevisiae is distinct from the vertebrate enzyme, suggesting that budding yeast and vertebrates may have evolved different mechanisms of CDK activation. |
| Keywords: | gene deletion; nonhuman; cell cycle; enzyme activation; enzyme activity; phosphorylation; enzyme phosphorylation; amino acid sequence; molecular sequence data; recombinant fusion proteins; saccharomyces cerevisiae; protein-serine-threonine kinases; yeast; cyclin-dependent kinases; cyclin dependent kinase; enzyme subunit; vertebrate; molecular weight; cell lysate; cyclin-dependent kinase 2; genes, fungal; cdc28 protein kinase, s cerevisiae; cdc2-cdc28 kinases; humans; priority journal; article |
| Journal Title: | Science |
| Volume: | 273 |
| Issue: | 5282 |
| ISSN: | 0036-8075 |
| Publisher: | American Association for the Advancement of Science |
| Date Published: | 1996-09-20 |
| Start Page: | 1714 |
| End Page: | 1717 |
| Language: | English |
| PUBMED: | 8781234 |
| PROVIDER: | scopus |
| DOI: | 10.1126/science.273.5282.1714 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 22 November 2017 -- Source: Scopus |
