Inducible in vivo genome editing with CRISPR-Cas9 Journal Article


Authors: Dow, L. E.; Fisher, J.; O'Rourke, K. P.; Muley, A.; Kastenhuber, E. R.; Livshits, G.; Tschaharganeh, D. F.; Socci, N. D.; Lowe, S. W.
Article Title: Inducible in vivo genome editing with CRISPR-Cas9
Abstract: CRISPR-Cas9-based genome editing enables the rapid genetic manipulation of any genomic locus without the need for gene targeting by homologous recombination. Here we describe a conditional transgenic approach that allows temporal control of CRISPR-Cas9 activity for inducible genome editing in adult mice. We show that doxycycline-regulated Cas9 induction enables widespread gene disruption in multiple tissues and that limiting the duration of Cas9 expression or using a Cas9<inf>D10A</inf> (Cas9n) variant can regulate the frequency and size of target gene modifications, respectively. Further, we show that this inducible CRISPR (iCRISPR) system can be used effectively to create biallelic mutation in multiple target loci and, thus, provides a flexible and fast platform to study loss-of-function phenotypes in vivo.
Keywords: homologous recombination; gene targeting; mus; genes; gene expression; plants (botany); biallelic mutations; gene disruptions; genetic manipulations; multiple targets; temporal controls; transgenic approaches
Journal Title: Nature Biotechnology
Volume: 33
Issue: 4
ISSN: 1087-0156
Publisher: Nature Publishing Group  
Date Published: 2015-04-01
Start Page: 390
End Page: 394
Language: English
DOI: 10.1038/nbt.3155
PROVIDER: scopus
PMCID: PMC4390466
PUBMED: 25690852
DOI/URL:
Notes: Export Date: 4 May 2015 -- Source: Scopus
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  1. Nicholas D Socci
    266 Socci
  2. Scott W Lowe
    249 Lowe
  3. Lukas Dow
    18 Dow
  4. Jonathan Ming-Yan Fisher
    1 Fisher