CRISPR/Cas9-based engineering of the epigenome Journal Article
| Authors: | Pulecio, J.; Verma, N.; Mejía-Ramírez, E.; Huangfu, D.; Raya, A. |
| Article Title: | CRISPR/Cas9-based engineering of the epigenome |
| Abstract: | Determining causal relationships between distinct chromatin features and gene expression, and ultimately cell behavior, remains a major challenge. Recent developments in targetable epigenome-editing tools enable us to assign direct transcriptional and functional consequences to locus-specific chromatin modifications. This Protocol Review discusses the unprecedented opportunity that CRISPR/Cas9 technology offers for investigating and manipulating the epigenome to facilitate further understanding of stem cell biology and engineering of stem cells for therapeutic applications. We also provide technical considerations for standardization and further improvement of the CRISPR/Cas9-based tools to engineer the epigenome. Pulecio et al. discuss opportunities that CRISPR/Cas9 technology offers for investigating and manipulating the stem cell epigenome and also provide technical considerations for CRISPR/Cas9 tool standardization and improvement. © 2017 Elsevier Inc. |
| Keywords: | promoter region; review; nonhuman; gene amplification; gene expression; dna methylation; stem cell; transcription regulation; epigenetics; chromatin; pluripotent stem cell; untranslated rna; eukaryote; transcriptional regulation; protein modification; demethylation; histone modification; histone modifications; cell engineering; chromatin architecture; human; priority journal; stem cell self-renewal; crispr-cas9 system; crispr-dcas9; targeted epigenome engineering; targeted gene edition; silencer gene |
| Journal Title: | Cell Stem Cell |
| Volume: | 21 |
| Issue: | 4 |
| ISSN: | 1934-5909 |
| Publisher: | Cell Press |
| Date Published: | 2017-10-05 |
| Start Page: | 431 |
| End Page: | 447 |
| Language: | English |
| DOI: | 10.1016/j.stem.2017.09.006 |
| PROVIDER: | scopus |
| PUBMED: | 28985525 |
| PMCID: | PMC6205890 |
| DOI/URL: | |
| Notes: | Review -- Export Date: 4 December 2017 -- Source: Scopus |
Altmetric
Citation Impact
BMJ Impact Analytics
MSK Authors
Related MSK Work