Femoral bone marrow aspiration in live mice Journal Article

   


Authors: Chung, Y. R.; Kim, E.; Abdel-Wahab, O.
Article Title: Femoral bone marrow aspiration in live mice
Abstract: Serial sampling of the cellular composition of bone marrow (BM) is a routine procedure critical to clinical hematology. This protocol describes a detailed step-by-step technical procedure for an analogous procedure in live mice which allows for serial characterization of cells present in the BM. This procedure facilitates studies aimed to detect the presence of exogenously administered cells within the BM of mice as would be done in xenograft studies for instance. Moreover, this procedure allows for the retrieval and characterization of cells enriched in the BM such as hematopoietic stem and progenitor cells (HSPCs) without sacrifice of mice. Given that the cellular composition of peripheral blood is not necessarily reflective of proportions and types of stem and progenitor cells present in the marrow, procedures which provide access to this compartment without requiring termination of the mice are very helpful. The use of femoral bone marrow aspiration is illustrated here for cytological analysis of marrow cells, flow cytometric characterization of the hematopoietic stem/progenitor compartment, and culture of sorted HSPCs obtained by femoral BM aspiration compared with conventional marrow harvest. © JoVE 2006-2014. All Rights Reserved.
Keywords: leukemia; bone marrow; aspiration; hematopoiesis; hematopoietic stem cell; medicine; mouse model; issue 89
Journal Title: Journal of Visualized Experiments
Issue: 89
ISSN: 1940-087X
Publisher: MYJoVE Corporation  
Date Published: 2014-07-05
Start Page: e51660
Language: English
DOI: 10.3791/51660
PROVIDER: scopus
PUBMED: 25045847
PMCID: PMC4211899
DOI/URL:

http://www.scopus.com/inward/record.url?eid=2-s2.0-84924219431&partnerID=40&md5=07560dce94169de877f8497b901a2e98
Notes: Export Date: 2 April 2015 -- Source: Scopus
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MSK Authors
  1. Omar Ibrahim Abdel-Wahab
    573 Abdel-Wahab
  2. Young Rock Chung
    48 Chung
  3. Eunhee Kim
    29 Kim
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