Expression of a functional eotaxin (CC chemokine ligand 11) receptor CCR3 by human dendritic cells Journal Article
| Authors: | Beaulieu, S.; Robbiani, D. F.; Du, X. X.; Rodrigues, E.; Ignatius, R.; Wei, Y.; Ponath, P.; Young, J. W.; Pope, M.; Steinman, R. M.; Mojsov, S. |
| Article Title: | Expression of a functional eotaxin (CC chemokine ligand 11) receptor CCR3 by human dendritic cells |
| Abstract: | Critical to the function of Ag-presenting dendritic cells (DCs) is their capacity to migrate to lymphoid organs and to sites of inflammation. A final stage of development, termed maturation, yields DCs that are strong stimulators of T cell-mediated immunity and is associated with a remodeling of the cell surface that includes a change in the levels of expression of many molecules, including chemokine receptors. We show in this study that CCR3, a chemokine receptor initially discovered on eosinophils, is also expressed by human DCs that differentiate from blood monocytes, DCs that emigrate from skin (epidermal and dermal DCs), and DCs derived from CD34(+) hemopoietic precursors in bone marrow, umbilical cord blood, and cytokine-elicited peripheral blood leukapheresis. Unlike other chemokine receptors, such as CCR5 and CCR7, the expression of CCR3 is not dependent on the state of maturation. All DC subsets contain a large intracellular pool of CCR3. The surface expression of CCR3 is not modulated following uptake of particulate substances such as zymosan or latex beads. CCR3 mediates in vitro chemotactic responses to the known ligands, eotaxin and eotaxin-2, because the DC response to these chemokines is inhibited by CCR3-specific mAbs. We postulate that expression of CCR3 may underlie situations where both DCs and eosinophils accumulate in vivo, such as the lesions of patients with Langerhans cell granulomatosis. |
| Keywords: | t-lymphocytes; human skin; colony-stimulating factor; progenitors; human blood; cd34(+) hematopoietic; human langerhans cells; human eosinophil; selective recruitment; natural antagonist; human monocyte |
| Journal Title: | Journal of Immunology |
| Volume: | 169 |
| Issue: | 6 |
| ISSN: | 0022-1767 |
| Publisher: | The American Association of Immunologists, Inc |
| Date Published: | 2002-09-15 |
| Start Page: | 2925 |
| End Page: | 2936 |
| Language: | English |
| ACCESSION: | WOS:000177958200018 |
| PROVIDER: | wos |
| PUBMED: | 12218106 |
| DOI: | 10.4049/jimmunol.169.6.2925 |
| Notes: | Parts of this work were presented at the 6th International Workshop on Langerhans Cells, 1999 Oct 8–10, New York, NY; the 14th Spring Meeting of the Canadian Society for Immunology, 2000 Mar 17–20, Bromont, Canada; and the 9th Annual Canadian Conference on HIV/AIDS Research, 2000 Apr 27–30, Montréal, Canada -- Article; Proceedings Paper -- Source: Wos |
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