Generation of neuropeptidergic hypothalamic neurons from human pluripotent stem cells Journal Article
| Authors: | Merkle, F. T.; Maroof, A.; Wataya, T.; Sasai, Y.; Studer, L.; Eggan, K.; Schier, A. F. |
| Article Title: | Generation of neuropeptidergic hypothalamic neurons from human pluripotent stem cells |
| Abstract: | Hypothalamic neurons orchestrate many essential physiological and behavioral processes via secreted neuropeptides, and are relevant to human diseases such as obesity, narcolepsy and infertility. We report the differentiation of human pluripotent stem cells into many of the major types of neuropeptidergic hypothalamic neurons, including those producing pro-opiolemelanocortin, agoutirelated peptide, hypocretin/orexin, melanin-concentrating hormone, oxytocin, arginine vasopressin, corticotropin-releasing hormone (CRH) or thyrotropin-releasing hormone. Hypothalamic neurons can be generated using a ‘self-patterning’ strategy that yields a broad array of cell types, or via a more reproducible directed differentiation approach. Stem cell-derived human hypothalamic neurons share characteristic morphological properties and gene expression patterns with their counterparts in vivo, and are able to integrate into the mouse brain. These neurons could form the basis of cellular models, chemical screens or cellular therapies to study and treat common human diseases. |
| Keywords: | protein kinase b; adolescent; adult; controlled study; protein expression; unclassified drug; human cell; nonhuman; animal cell; mouse; animal tissue; cell death; cell survival; cell division; cell structure; embryonic stem cell; glial fibrillary acidic protein; animal experiment; brain cortex; transcription factor; neural stem cell; cell differentiation; in vitro study; cell population; stem cell; tyrosine 3 monooxygenase; nerve fiber; hypothalamus; glia cell; cell aggregation; pluripotent; pluripotent stem cell; transcription factor foxg1; forebrain; rhombencephalon; nerve cell; neuropeptide; differentiation; telencephalon; narcolepsy; transcription factor nkx2.1; 4 aminobutyric acid; corticotropin releasing factor; melanin concentrating hormone; orexin; oxytocin; proopiomelanocortin; dynorphin a; neuroepithelium; xenotransplantation; pentraxin; human; priority journal; article; argipressin; growth hormone releasing factor; kisspeptin; transcription factor otp; transcription factor rax; transcription factor sim1; neuropeptidergic hypothalamic neuron |
| Journal Title: | Development |
| Volume: | 142 |
| Issue: | 4 |
| ISSN: | 0950-1991 |
| Publisher: | Company of Biologists |
| Date Published: | 2015-02-15 |
| Start Page: | 633 |
| End Page: | 643 |
| Language: | English |
| DOI: | 10.1242/dev.117978 |
| PROVIDER: | scopus |
| PUBMED: | 25670790 |
| PMCID: | PMC4325380 |
| DOI/URL: | |
| Notes: | Export Date: 2 March 2015 -- Source: Scopus |
