Expression profiling of lineage differentiation in pluripotential human embryonal carcinoma cells Journal Article

  


Authors: Houldsworth, J.; Heath, S. C.; Bosl, G. J.; Studer, L.; Chaganti, R. S. K.
Article Title: Expression profiling of lineage differentiation in pluripotential human embryonal carcinoma cells
Abstract: Pluripotential human embryonal carcinoma (EC) cell lines undergo differentiation programs resembling those occurring in embryonal stem cells during development. Expression profiling was performed during the terminal differentiation of the EC cell line, NTera2/Clone D1 by all-trans-retinoic acid. Time-response analysis via clustering of > 12,000 human transcripts revealed distinct stages in the transition from an EC cell to neuronal progenitor cells expressing patterning markers compatible with posterior hindbrain fates followed by the appearance of immature postmitotic neurons with an evolving synaptic apparatus. Global analysis of gene expression allows monitoring cell fate and differentiation of EC cells in vitro and may provide insight into human embryonal stem cell development.
Keywords: human cell; genetics; antineoplastic agents; antineoplastic agent; cell division; gene expression; gene expression profiling; cell maturation; embryo; cell line; membrane proteins; cell fate; embryo development; cell differentiation; neurons; drug effect; pathology; carcinogenesis; cell lineage; stem cell; rna; gene expression regulation; gene expression regulation, developmental; messenger rna; rna, messenger; membrane protein; cancer stem cell; blotting, northern; nerve cell differentiation; nerve cell; retinoic acid; embryonal carcinoma; carcinoma cell; northern blotting; rna analysis; tretinoin; tumor stem cells; humans; human; priority journal; article; support, non-u.s. gov't; support, u.s. gov't, p.h.s.
Journal Title: Cell Growth & Differentiation
Volume: 13
Issue: 6
ISSN: 1044-9523
Publisher: American Association for Cancer Research  
Date Published: 2002-06-01
Start Page: 257
End Page: 264
Language: English
PUBMED: 12114215
PROVIDER: scopus
DOI/URL:

http://www.scopus.com/inward/record.url?eid=2-s2.0-0035989923&partnerID=40&md5=4012b8063cef520c1237cb86c3c48fbf
Notes: Export Date: 14 November 2014 -- Source: Scopus
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MSK Authors
  1. Simon C Heath
    16 Heath
  2. Lorenz Studer
    223 Studer
  3. Raju S K Chaganti
    391 Chaganti
  4. George Bosl
    430 Bosl
  5. Jane Houldsworth El Naggar
    101 Houldsworth El Naggar
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