Adaptive regulation of testis gene expression and control of male fertility by the drosophila harpin RNA pathway Journal Article

   

Authors:	Wen, J.; Duan, H.; Bejarano, F. ; Okamura, K.; Fabian, L.; Brill, J. A.; Bortolamiol-Becet, D.; Martin, R.; Ruby, J. G.; Lai, E. C.
Article Title:	Adaptive regulation of testis gene expression and control of male fertility by the drosophila harpin RNA pathway
Abstract:	Although endogenous siRNAs (endo-siRNAs) have been described in many species, still little is known about their endogenous utility. Here, we show that Drosophila hairpin RNAs (hpRNAs) generate an endo-siRNA class with predominant expression in testes. Although hpRNAs are universally recently evolved, we identify highly complementary protein-coding targets for all hpRNAs. Importantly, we find broad evidence for evolutionary divergences that preferentially maintain compensatory pairing between hpRNAs and targets, serving as first evidence for adaptive selection for siRNA-mediated target regulation in metazoans. We demonstrate organismal impact of hpRNA activity, since knockout of hpRNA1 derepresses its target ATP synthase-β in testes and compromises spermatogenesis and male fertility. Moreover, we reveal surprising male-specific impact of RNAi factors on germ cell development and fertility, consistent with testis-directed function of the hpRNA pathway. Finally, the collected hpRNA loci chronicle an evolutionary timeline that reflects their origins from prospective target genes, mirroring a strategy described for plant miRNAs.
Keywords:	signal transduction; adult; controlled study; nonhuman; animal cell; phenotype; animal; animal tissue; gene targeting; cell maturation; embryo; germ cell; animal experiment; small interfering rna; gene locus; drosophila; evolution; gene expression regulation; genetic code; testis; short hairpin rna; male fertility; metazoa; spermatogenesis; proton transporting adenosine triphosphate synthase; male; article; organismal interaction
Journal Title:	Molecular Cell
Volume:	57
Issue:	1
ISSN:	1097-2765
Publisher:	Cell Press
Date Published:	2015-01-08
Start Page:	165
End Page:	178
Language:	English
DOI:	10.1016/j.molcel.2014.11.025
PROVIDER:	scopus
PMCID:	PMC4289472
PUBMED:	25544562
DOI/URL:	http://www.scopus.com/inward/record.url?eid=2-s2.0-84920565409&partnerID=40&md5=46f30394f0f35002ec96fe85a1ed57de
Notes:	Export Date: 2 February 2015 -- Source: Scopus

https://synapse.mskcc.org/synapse/works/has_related_data_chk/67235