The Drosophila hairpin RNA pathway generates endogenous short interfering RNAs Journal Article

Authors: Okamura, K.; Chung, W. J.; Ruby, J. G.; Guo, H.; Bartel, D. P.; Lai, E. C.
Article Title: The Drosophila hairpin RNA pathway generates endogenous short interfering RNAs
Abstract: In contrast to microRNAs and Piwi-associated RNAs, short interfering RNAs (siRNAs) are seemingly dispensable for host-directed gene regulation in Drosophila. This notion is based on the fact that mutants lacking the core siRNA-generating enzyme Dicer-2 or the predominant siRNA effector Argonaute 2 are viable, fertile and of relatively normal morphology. Moreover, endogenous Drosophila siRNAs have not yet been identified. Here we report that siRNAs derived from long hairpin RNA genes (hpRNAs) programme Slicer complexes that can repress endogenous target transcripts. The Drosophila hpRNA pathway is a hybrid mechanism that combines canonical RNA interference factors (Dicer-2, Hen1 (known as CG12367) and Argonaute 2) with a canonical microRNA factor (Loquacious) to generate ∼21-nucleotide siRNAs. These novel regulatory RNAs reveal unexpected complexity in the sorting of small RNAs, and open a window onto the biological usage of endogenous RNA interference in Drosophila. ©2008 Nature Publishing Group.
Keywords: genetics; mutation; nonhuman; animals; gene expression; biology; protein; small interfering rna; rna, small interfering; drosophila; rna interference; morphology; rna; rna-binding proteins; methyltransferases; transcription regulation; fly; drosophila melanogaster; nucleic acid conformation; micrornas; drosophila proteins; rna helicases; rna-induced silencing complex; argonaute 2 protein; rna, double-stranded; hybrid; identification method; dicer 2 protein; hen1 protein
Journal Title: Nature
Volume: 453
Issue: 7196
ISSN: 0028-0836
Publisher: Nature Publishing Group  
Date Published: 2008-06-05
Start Page: 803
End Page: 806
Language: English
DOI: 10.1038/nature07015
PUBMED: 18463630
PROVIDER: scopus
PMCID: PMC2735555
Notes: --- - "Cited By (since 1996): 115" - "Export Date: 17 November 2011" - "CODEN: NATUA" - "Source: Scopus"
Citation Impact
MSK Authors
  1. Eric C Lai
    141 Lai
  2. Katsutomo Okamura
    26 Okamura
  3. Weijen Chung
    9 Chung