Dicing of viral replication intermediates during silencing of latent Drosophila viruses Journal Article

Authors: Flynt, A.; Liu, N.; Martin, R.; Lai, E. C.
Article Title: Dicing of viral replication intermediates during silencing of latent Drosophila viruses
Abstract: Previous studies revealed roles for RNA interference (RNAi) in the immediate cellular response to viral infection in plants, nematodes and flies. However, little is known about how RNAi combats viruses during persistent or latent infections. Our analysis of small RNAs cloned from Drosophila cells latently infected with Flock House Virus (FHV) failed to reveal signatures of bulk degradation of the viral genome. Instead, this + strand virus specifically generated Dicer-2-dependent, 21-nucleotidesiRNAs that derived in equal proportion from + and - strands. Curiously, luciferase reporters that are fully complementary to abundant viral siRNAs were poorly repressed. Moreover, although the viral siRNAs that were incorporated into an effector complex associated with Argonaute2, bulk FHV siRNAs in latently infected cells were not loaded into any Argonaute protein. Together, these data suggest that direct dicing of viral replication intermediates plays an important role in maintaining the latent viral state. In addition, the denial of bulk viral siRNAs from effector complexes suggests that criteria beyond the structural competency of RNA duplexes influence the assembly of functional silencing complexes.
Keywords: controlled study; nonhuman; animals; complex formation; cell line; small interfering rna; rna, small interfering; drosophila; rna interference; virus infection; virus replication; argonaute2; dicer-2; virus; argonaute protein; flock house virus; virus genome; virus strain; drosophila proteins; rna helicases; rna-induced silencing complex; virus latency; nematoda
Journal Title: Proceedings of the National Academy of Sciences of the United States of America
Volume: 106
Issue: 13
ISSN: 0027-8424
Publisher: National Academy of Sciences  
Date Published: 2009-03-31
Start Page: 5270
End Page: 5275
Language: English
DOI: 10.1073/pnas.0813412106
PUBMED: 19251644
PROVIDER: scopus
PMCID: PMC2663985
Notes: --- - "Cited By (since 1996): 10" - "Export Date: 30 November 2010" - "CODEN: PNASA" - "Source: Scopus"
Citation Impact
MSK Authors
  1. Na Liu
    5 Liu
  2. Eric C Lai
    146 Lai
  3. Alex S Flynt
    12 Flynt