Selective photostimulation of genetically chARGed neurons Journal Article

  


Authors: Zemelman, B. V.; Lee, G. A.; Ng, M.; Miesenboeck, G.
Article Title: Selective photostimulation of genetically chARGed neurons
Abstract: To permit direct functional analyses of neural circuits, we have developed a method for stimulating groups of genetically designated neurons optically. Coexpression of the Drosophila photoreceptor genes encoding arrestin-2, rhodopsin (formed by liganding opsin with retinal), and the α subunit of the cognate heterotrimeric G protein - an explosive combination we term "chARGe" - sensitizes generalist vertebrate neurons to light. Illumination of a mixed population of neurons elicits action potentials selectively and cell-autonomously in its genetically chARGed members. In contrast to bath-applied photostimulants or caged neurotransmitters, which act indiscriminately throughout the illuminated volume, chARGe localizes the responsiveness to light. Distributed activity may thus be fed directly into a circumscribed population of neurons in intact tissue, irrespective of the spatial arrangement of its elements.
Keywords: controlled study; nonhuman; animal cell; animals; oocytes; animal tissue; cells, cultured; gene expression; gtp-binding proteins; eye proteins; cell population; rna, messenger; nucleotide sequence; rat; phosphoproteins; retina; rats; fetus; photic stimulation; alpha chain; guanine nucleotide binding protein; nerve cell; drosophila proteins; genetic code; photosensitivity; transgenes; electric activity; nerve potential; xenopus; neural pathways; photostimulation; photoreceptor; hippocampus; light; illumination; nerve cell stimulation; arrestins; action potentials; female; priority journal; article; phototransduction; retina s antigen; rhodopsin; photoreceptors, invertebrate; retinaldehyde
Journal Title: Neuron
Volume: 33
Issue: 1
ISSN: 0896-6273
Publisher: Cell Press  
Date Published: 2002-01-03
Start Page: 15
End Page: 22
Language: English
DOI: 10.1016/s0896-6273(01)00574-8
PUBMED: 11779476
PROVIDER: scopus
DOI/URL:

http://www.scopus.com/inward/record.url?eid=2-s2.0-0037012096&partnerID=40&md5=306e70132035d4bf64dc14df1ea56991
Notes: Export Date: 14 November 2014 -- Source: Scopus
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MSK Authors
  1. Gero A Miesenboeck
    17 Miesenboeck
  2. Boris V Zemelman
    8 Zemelman
  3. Georgia Lee
    2 Lee
  4. Minna Ng
    2 Ng
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