High-density cell microarrays for parallel functional determinations Journal Article
| Author: | Xu, C. W. |
| Article Title: | High-density cell microarrays for parallel functional determinations |
| Abstract: | Whole-genome sequencing projects have generated a wealth of gene sequences from a variety of organisms. A major challenge is to rapidly uncover gene regulatory circuits and their functional manifestations at the cellular level. Here we report the coupled fabrication of nanocraters ranging in size from 100 pL to 1.5 nL on permeable membranes for culturing cells. Using this approach, we developed bacterial and yeast cell microarrays that allowed phenotypic determinations of gene activities and drug targets on a large scale. Cell microarrays will therefore be a particularly useful tool for studying phenotypes of gene activities on a genome-wide scale. |
| Keywords: | controlled study; gene sequence; nonhuman; phenotype; gene targeting; gene function; bacteria (microorganisms); regulatory mechanism; cell culture; saccharomyces cerevisiae; escherichia coli; dna microarray; bacteriological techniques; yeast cell; membranes, artificial; nanotechnology; gene activity; cell membrane permeability; genome, fungal; genetic regulation; bacterial cell; genes, fungal; genome, bacterial; genes, bacterial; mycology; priority journal; article |
| Journal Title: | Genome Research |
| Volume: | 12 |
| Issue: | 3 |
| ISSN: | 1088-9051 |
| Publisher: | Cold Spring Harbor Laboratory Press |
| Date Published: | 2002-03-01 |
| Start Page: | 482 |
| End Page: | 486 |
| Language: | English |
| DOI: | 10.1101/gr.213002 |
| PUBMED: | 11875037 |
| PROVIDER: | scopus |
| PMCID: | PMC155288 |
| DOI/URL: | |
| Notes: | Export Date: 14 November 2014 -- Source: Scopus |
