The topoisomerase I-binding RING protein, topors, is associated with promyelocytic leukemia nuclear bodies Journal Article

  

Authors:	Rasheed, Z. A.; Saleem, A.; Ravee, Y.; Pandolfi, P. P.; Rubin, E. H.
Article Title:	The topoisomerase I-binding RING protein, topors, is associated with promyelocytic leukemia nuclear bodies
Abstract:	We previously identified topors as a topoisomerase I-binding protein that localizes in punctate nuclear regions when expressed as a GFP fusion protein. We now demonstrate that both the GFP-topors fusion protein and endogenous topors are associated with promyelocytic leukemia (PML) nuclear bodies in exponentially growing HeLa cells. Studies using isogenic PML+/+ and PML-/- murine embryonic fibroblasts indicate that the punctate nuclear localization of topors is dependent on PML. A basic C-terminal region but not the N-terminal RING domain of topors is required for the punctate nuclear localization of this protein. Additional studies indicate that topors, but not PML, rapidly relocalizes from nuclear bodies to the nucleoplasm in cells exposed to the transcription inhibitor dichloro-1-β-D-ribofuranolsylbenzimidazole or to the topoisomerase I-targeting drug camptothecin. These results identify topors as a new member of the group of proteins that associate dynamically with PML nuclear bodies and suggest that topors may be involved in the cellular response to camptothecin. © 2002 Elsevier Science (USA).
Keywords:	controlled study; unclassified drug; human cell; dna-binding proteins; nonhuman; protein localization; animal cell; mouse; animals; mice; cell growth; carboxy terminal sequence; neoplasm proteins; camptothecin; hela cell; hela cells; transcription factors; nuclear proteins; leukemia, promyelocytic, acute; dimethyl sulfoxide; amino acid sequence; molecular sequence data; sequence homology, amino acid; hybrid protein; amino terminal sequence; recombinant fusion proteins; leukemia cell; carrier proteins; tumor suppressor proteins; drug response; promyelocytic leukemia; murinae; fibroblast; cytoplasm; binding protein; binding sites; cell nucleus; drug sensitivity; protein inhibitor; drug exposure; chromosome mapping; dna topoisomerase; dna topoisomerases, type i; embryo cell; zinc fingers; rabbits; nucleic acid synthesis inhibitors; cell nucleus inclusion body; humans; human; priority journal; article; dichloro 1 beta dextro ribofuranosylbenzimidazole; protein topors; dichlororibofuranosylbenzimidazole
Journal Title:	Experimental Cell Research
Volume:	277
Issue:	2
ISSN:	0014-4827
Publisher:	Elsevier Inc.
Date Published:	2002-07-15
Start Page:	152
End Page:	160
Language:	English
DOI:	10.1006/excr.2002.5550
PUBMED:	12083797
PROVIDER:	scopus
DOI/URL:	http://www.scopus.com/inward/record.url?eid=2-s2.0-0036314919&partnerID=40&md5=e1feb079e55deca3b1e9f47915738ece
Notes:	Export Date: 14 November 2014 -- Source: Scopus

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