Characterization and expression of mammalian cyclin B3, a prepachytene meiotic cyclin Journal Article
| Authors: | Nguyen, T. B.; Manova, K.; Capodieci, P.; Lindon, C.; Bottega, S.; Wang, X. Y.; Refik Rogers, J.; Pines, J.; Wolgemuth, D. J.; Koff, A. |
| Article Title: | Characterization and expression of mammalian cyclin B3, a prepachytene meiotic cyclin |
| Abstract: | We report the identification and expression pattern of a full-length human cDNA and a partial mouse cDNA encoding cyclin B3. Cyclin B3 (CCNB3) is conserved from Caenorhabditis elegans to Homo sapiens and has an undefined meiotic function in female, but not male Drosophila melanogaster. We show that H. sapiens cyclin B3 interacts with cdk2, is localized to the nucleus, and is degraded during anaphase entry after the degradation of cyclin B1. Degradation is dependent on sequences conserved in a destruction box motif. Overexpression of nondegradable cyclin B3 blocks the mitotic cell cycle in late anaphase, and at higher doses it can interfere with progression through G1 and entry into S phase. H. sapiens cyclin B3 mRNA and protein are detected readily in developing germ cells in the human testis and not in any other tissue. The mouse cDNA has allowed us to further localize cyclin B3 mRNA to leptotene and zygotene spermatocytes. The expression pattern of mammalian cyclin B3 suggests that it may be important for events occurring in early meiotic prophase I. |
| Keywords: | controlled study; unclassified drug; human cell; nonhuman; protein localization; protein motif; mitosis; spermatocyte; meiosis; mammalia; animals; mice; cell cycle; cell cycle s phase; gene overexpression; gene expression; germ cell; protein degradation; protein protein interaction; gene function; hela cells; rna; ovary; dna; molecular sequence data; messenger rna; rna, messenger; gene identification; nucleotide sequence; cellular distribution; base sequence; anaphase; caenorhabditis elegans; drosophila melanogaster; cell nucleus; fetus; cyclin b1; cyclins; cyclin b; mammals; drosophila proteins; genetic code; testis; genetic conservation; cell cycle g1 phase; enzymes; complementary dna; tissue; g2 phase; prophase; cells; spermatogenesis; biodegradation; melanogaster; caenorhabditis; cyclin b3; homo sapiens; homo; humans; human; male; female; priority journal; article; non-programmatic |
| Journal Title: | Journal of Biological Chemistry |
| Volume: | 277 |
| Issue: | 44 |
| ISSN: | 0021-9258 |
| Publisher: | American Society for Biochemistry and Molecular Biology |
| Date Published: | 2002-11-01 |
| Start Page: | 41960 |
| End Page: | 41969 |
| Language: | English |
| DOI: | 10.1074/jbc.M203951200 |
| PUBMED: | 12185076 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Export Date: 14 November 2014 -- Source: Scopus |
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