Cyclin B3 is dispensable for mouse spermatogenesis Journal Article

Authors: Karasu, M. E.; Keeney, S.
Article Title: Cyclin B3 is dispensable for mouse spermatogenesis
Abstract: Cyclins, as regulatory partners of cyclin-dependent kinases (CDKs), control the switch-like cell cycle transitions that orchestrate orderly duplication and segregation of genomes. Compared to mitosis, relatively little is known about how cyclin-CDK complexes control meiosis, the specialized cell division that generates gametes for sexual production. Mouse cyclin B3 was previously shown to have expression restricted to the beginning of meiosis, making it a candidate to regulate meiotic events. Indeed, female mice lacking cyclin B3 are sterile because oocytes arrest at the metaphase-to-anaphase transition of meiosis I. However, whether cyclin B3 functions during spermatogenesis was untested. Here, we found that males lacking cyclin B3 are fertile and show no detectable defects in spermatogenesis based on histological analysis of seminiferous tubules. Cytological analysis further showed no detectable defects in homologous chromosome synapsis or meiotic progression, and suggested that recombination is initiated and completed efficiently. Moreover, absence of cyclin B3 did not exacerbate previously described meiotic defects in mutants deficient for cyclin E2, suggesting a lack of redundancy between these cyclins. Thus, unlike in females, cyclin B3 is not essential for meiosis in males despite its prominent meiosis-specific expression. © 2019, Springer-Verlag GmbH Germany, part of Springer Nature.
Keywords: controlled study; unclassified drug; nonhuman; animal cell; chromosome; mouse; oocyte; meiosis; animal tissue; gene expression; animal experiment; cyclin b; cyclin dependent kinase; chromosome pairing; cyclin e; recombination; spermatogenesis; cyclin; cyclin b3; seminiferous tubule; cyclin e2; male; article
Journal Title: Chromosoma
Volume: 128
Issue: 3
ISSN: 0009-5915
Publisher: Springer  
Date Published: 2019-09-01
Start Page: 473
End Page: 487
Language: English
DOI: 10.1007/s00412-019-00725-5
PUBMED: 31446450
PROVIDER: scopus
PMCID: PMC6824995
Notes: Article -- Export Date: 2 December 2019 -- Source: Scopus
Citation Impact
MSK Authors
  1. Scott N Keeney
    113 Keeney
  2. Mehmet Erman Karasu
    5 Karasu