Differential presentation of a soluble exogenous tumor antigen, NY-ESO-1, by distinct human dendritic cell populations Journal Article
| Authors: | Nagata, Y.; Ono, S.; Matsuo, M.; Gnjatic, S.; Valmori, D.; Ritter, G.; Garrett, W.; Old, L. J.; Mellman, I. |
| Article Title: | Differential presentation of a soluble exogenous tumor antigen, NY-ESO-1, by distinct human dendritic cell populations |
| Abstract: | Dendritic cells (DCs) play a critical role in initiating antigen-specific immune responses, because they are able to capture exogenous antigens for presentation to naïve T cells on both MHC class I and II molecules. As such, DCs represent important elements in the development of vaccine therapy for cancer. Although DCs are known to present antigens from phagocytosed tumor cells or preprocessed peptides, we explored whether they might also present soluble recombinant NY-ESO-1, a well characterized cancer antigen. We compared the abilities of human monocyte-derived DCs and DCs derived in vitro from CD34-positive stem cells to present NY-ESO-1 epitopes to MHC class I-restricted cytotoxic T cells. Although monocyte-derived DCs did not efficiently cross-present free NY-ESO-1 protein, IgG-immune complexes containing NY-ESO-1 were avidly presented after uptake by Fcγ receptors (FcγRII). In contrast, CD34-derived DCs were unable to process either soluble or immune complexed NY-ESO-1, although they efficiently presented preprocessed NY-ESO-1 peptides. This difference did not necessarily correlate with endocytic capacity. Although monocyte-derived DCs exhibited greater fluid-phase uptake than CD34-derived DCs, the two populations did not differ with respect to their surprisingly limited capacity for Fcγ receptor-mediated endocytosis. These results indicate that monocyte-derived DCs will be easier to load by using protein antigen in vitro than CD34-derived DCs, and that the latter population exhibits a restricted ability to crosspresent soluble exogenous antigens. |
| Keywords: | controlled study; unclassified drug; human cell; proteins; cells, cultured; complex formation; cd34 antigen; cancer immunotherapy; dendritic cell; membrane proteins; cell population; tumor antigen; stem cell; antigen presentation; dendritic cells; immune response; antigens, neoplasm; cancer vaccine; ny eso 1 antigen; recombinant antigen; cytotoxic t lymphocyte; stem cells; fc receptor; immunophenotyping; monocyte; monocytes; phagocytosis; macrophages; major histocompatibility complex; endocytosis; antigens, cd34; immunoglobulin g antibody; major histocompatibility antigen class 1; solubility; antigen antibody complex; humans; human; priority journal; article |
| Journal Title: | Proceedings of the National Academy of Sciences of the United States of America |
| Volume: | 99 |
| Issue: | 16 |
| ISSN: | 0027-8424 |
| Publisher: | National Academy of Sciences |
| Date Published: | 2002-08-06 |
| Start Page: | 10629 |
| End Page: | 10634 |
| Language: | English |
| DOI: | 10.1073/pnas.112331099 |
| PUBMED: | 12138174 |
| PROVIDER: | scopus |
| PMCID: | PMC124995 |
| DOI/URL: | |
| Notes: | Export Date: 14 November 2014 -- Source: Scopus |
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