Germinal center B cells regulate their capability to present antigen by modulation of HLA-DO Journal Article
| Authors: | Glazier, K. S.; Hake, S. B.; Tobin, H. M.; Chadburn, A.; Schattner, E. J.; Denzin, L. K. |
| Article Title: | Germinal center B cells regulate their capability to present antigen by modulation of HLA-DO |
| Abstract: | Peptide acquisition by MHC class II molecules is catalyzed by HLA-DM (DM). In B cells, HLA-DO (DO) inhibits or modifies the peptide exchange activity of DM. We show here that DO protein levels are modulated during B cell differentiation. Remarkably, germinal center (GC) B cells, which have low levels of DO relative to naive and memory B cells, are shown to have enhanced antigen presentation capabilities. DM protein levels also were somewhat reduced in GC B cells; however, the ratio of DM to DO in GC B cells was substantially increased, resulting in more free DM in GC B cells. We conclude that modulation of DM and DO in distinct stages of B cell differentiation represents a mechanism by which B cells regulate their capacity to function as antigen-presenting cells. Efficient antigen presentation in GC B cells would promote GC B cell-T cell interactions that are essential for B cells to survive positive selection in the GC. |
| Keywords: | controlled study; human cell; nonhuman; t lymphocyte; animal cell; mouse; cell survival; cell line; down-regulation; b-lymphocytes; germinal center; antigen presentation; major histocompatibility antigen class 2; hla-dr antigens; histocompatibility antigens class ii; antigen processing; hla dm antigen; hla do antigen; hla-d antigens; cell interaction; antigen presenting cell; antigen-presenting cells; b lymphocyte differentiation; hla-do; hla-dm; antigens, differentiation, b-lymphocyte; humans; human; priority journal; article; b-lymphocyte subsets; mhc class ii; germinal center b cells |
| Journal Title: | Journal of Experimental Medicine |
| Volume: | 195 |
| Issue: | 8 |
| ISSN: | 0022-1007 |
| Publisher: | Rockefeller University Press |
| Date Published: | 2002-04-15 |
| Start Page: | 1063 |
| End Page: | 1069 |
| Language: | English |
| DOI: | 10.1084/jem.20012059 |
| PUBMED: | 11956297 |
| PROVIDER: | scopus |
| PMCID: | PMC2193692 |
| DOI/URL: | |
| Notes: | Export Date: 14 November 2014 -- Source: Scopus |
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