p63 expression profiles in human normal and tumor tissues Journal Article

Authors: Di Como, C.; Urist, M. J.; Babayan, I.; Drobnjak, M.; Hedvat, C. V.; Teruya-Feldstein, J.; Pohar, K.; Hoos, A.; Cordon-Cardo, C.
Article Title: p63 expression profiles in human normal and tumor tissues
Abstract: Purpose: The p63 gene, located on chromosome 3q27-28, is a member of the p53 gene family. The product encoded by the p63 gene has been reported to be essential for normal development. Experimental Design: In this study, we examined the expression pattern of p63 in human normal and tumor tissues by immunohistochemistry using a monoclonal antibody (clone 4A4) that recognizes all p63 splice variants, and by reverse transcription-PCR using isoform-specific primers. Results: We found that p63 expression was restricted to the nucleus, with a nucleoplasmic pattern. We also observed that the expression was restricted to epithelial cells of stratified epithelia, such as skin, esophagus, exocervix, tonsil, and bladder, and to certain subpopulations of basal cells in glandular structures of prostate and breast, as well as in bronchi. Consistent with the phenotype observed in normal tissues, we found that p63 is expressed predominantly in basal cell and squamous cell carcinomas, as well as transitional cell carcinomas, but not in adenocarcinomas, including those of breast and prostate. Interestingly, thymomas expressed high levels of p63. Moreover, a subset of non-Hodgkin's lymphoma was also found to express p63. Using isoform-specific reverse transcription-PCR, we found that thymomas express all isoforms of p63, whereas the non-Hodgkin's lymphoma tended to express the transactivation-competent isoforms. We did not detect p63 expression in a variety of endocrine tumors, germ cell neoplasms, or melanomas. Additionally, soft tissue sarcomas were also found to have undetectable p63 levels. Conclusions: Our data support a role for p63 in squamous and transitional cell carcinomas, as well as certain lymphomas and thymomas.
Keywords: immunohistochemistry; human tissue; protein expression; major clinical study; dna-binding proteins; squamous cell carcinoma; neoplasms; adenocarcinoma; reverse transcription polymerase chain reaction; basal cell carcinoma; membrane proteins; tumor cells, cultured; hodgkin disease; time factors; monoclonal antibody; rna; tumor suppressor gene; b cell lymphoma; tissue distribution; thymus gland; reverse transcriptase polymerase chain reaction; nucleotide sequence; breast carcinoma; tumor suppressor proteins; lymphoma; alternative splicing; phosphoproteins; immunophenotyping; protein p63; trans-activators; thymoma; transitional cell carcinoma; protein isoforms; genes, tumor suppressor; prostate carcinoma; multigene family; humans; human; priority journal; article
Journal Title: Clinical Cancer Research
Volume: 8
Issue: 2
ISSN: 1078-0432
Publisher: American Association for Cancer Research  
Date Published: 2002-02-01
Start Page: 494
End Page: 501
Language: English
PUBMED: 11839669
PROVIDER: scopus
Notes: Export Date: 14 November 2014 -- Source: Scopus