T cells from the tumor microenvironment of patients with progressive myeloma can generate strong, tumor-specific cytolytic responses to autologous, tumor-loaded dendritic cells Journal Article


Authors: Dhodapkar, M. V.; Krasovsky, J.; Olson, K.
Article Title: T cells from the tumor microenvironment of patients with progressive myeloma can generate strong, tumor-specific cytolytic responses to autologous, tumor-loaded dendritic cells
Abstract: Most untreated cancer patients develop progressive tumors. We tested the capacity of T lymphocytes from patients with clinically progressive, multiple myeloma to develop killer function against fresh autologous tumor. In this malignancy, it is feasible to reproducibly evaluate freshly isolated tumor cells and T cells from the marrow tumor environment. When we did this with seven consecutive patients, with all clinical stages of disease, we did not detect reactivity to autologous cancer cells. However, both cytolytic and IFN-γ-producing responses to autologous myeloma were generated in six of seven patients after stimulation ex vivo with dendritic cells that had processed autologous tumor cells. The antitumor effectors recognized fresh autologous tumor but not nontumor cells in the bone marrow, myeloma cell lines, dendritic cells loaded with tumor-derived Ig, or allogeneic tumor. Importantly, these CD8 + effectors developed with similar efficiency by using T cells from both the blood and the bone marrow tumor environment. Therefore, even in the setting of clinical tumor progression, the tumor bed of myeloma patients contains T cells that can be activated readily by dendritic cells to kill primary autologous tumor.
Keywords: controlled study; human cell; cancer growth; cancer patient; cancer staging; t lymphocyte; cd8-positive t-lymphocytes; t-lymphocytes; bone marrow cells; cells, cultured; multiple myeloma; bone marrow; dendritic cell; cell line; immunoglobulin; tumor cells, cultured; cytokines; dendritic cells; gamma interferon; tumor cell; tumor immunity; tumor growth; vaccine; myeloma; tolerance; myeloma cell; killer cell; gammopathy; humans; human; priority journal; article; killer cells
Journal Title: Proceedings of the National Academy of Sciences of the United States of America
Volume: 99
Issue: 20
ISSN: 0027-8424
Publisher: National Academy of Sciences  
Date Published: 2002-10-01
Start Page: 13009
End Page: 13013
Language: English
DOI: 10.1073/pnas.202491499
PUBMED: 12235374
PROVIDER: scopus
PMCID: PMC130577
DOI/URL:
Notes: Export Date: 14 November 2014 -- Source: Scopus
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