Expression of SSX-2 and SSX-4 genes in neuroblastoma Journal Article
| Authors: | Chi, S. N.; Cheung, N. K. V.; Cheung, I. Y. |
| Article Title: | Expression of SSX-2 and SSX-4 genes in neuroblastoma |
| Abstract: | The SSX genes are members of the family of cancer/testis antigens that encode tumor-associated antigens recognizable by autologous cytolytic T lymphocytes. Their expression is common in tumors of diverse lineages and absent in normal tissues except testis and thyroid. In this study, sixty-seven neuroblastomas (NB) (12 stage 1, 13 stage 2, 12 stage 3, 12 stage 4S and 13 stage 4) were examined by RT-PCR and a sensitive chemiluminescent detection method for SSX-2 and SSX-4 expression. Seventy-two percent (13/18) of stage 4 NB expressed SSX-2 and 67% (12/18) expressed SSX-4. SSX-2 and SSX-4 positivity correlated with metastatic NB stage 4 (p=0.02 and p=0.006, respectively). Sensitivity experiments showed SSX-2 detection was one tumor cell in 106 normal cells, and one in 104 for SSX-4. All normal tissues (n=6), with the exception of testis, normal bone marrow (BM, n=12) and normal peripheral blood (PBL, n=10) were negative for SSX-2 and SSX-4 expression. Thirty-two BM and 14 PBL obtained from 35 stage 4 NB patients at 24 months from their diagnosis were evaluated for SSX-2 expression. Unlike another cancer/testis antigen, GAGE, only one BM sample was positive, and no prognostic utility could be established. Further investigation of SSX expression at other relevant time points is warranted. |
| Keywords: | controlled study; human tissue; human cell; major clinical study; cisplatin; doxorubicin; cancer staging; neoplasm staging; sensitivity and specificity; t lymphocyte; biological marker; metastasis; reverse transcription polymerase chain reaction; gene expression; bone marrow; etoposide; neoplasm proteins; tumor markers, biological; cyclophosphamide; vincristine; genetic transcription; tumor antigen; cell lineage; blood; correlation analysis; statistical analysis; antigens, neoplasm; tissue distribution; antigen specificity; neuroblastoma; reverse transcriptase polymerase chain reaction; tumor cell; cytolysis; cancer/testis antigen; cancer classification; thyroid gland; repressor proteins; testis; gene location; complementary dna; rna extraction; rt-pcr; tonsil; ssx; chemoluminescence; humans; prognosis; human; article |
| Journal Title: | International Journal of Biological Markers |
| Volume: | 17 |
| Issue: | 4 |
| ISSN: | 0393-6155 |
| Publisher: | Wichtig Editore |
| Date Published: | 2002-10-01 |
| Start Page: | 219 |
| End Page: | 223 |
| Language: | English |
| PUBMED: | 12521124 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Export Date: 14 November 2014 -- Source: Scopus |
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