Expression of SSX genes in human tumors Journal Article
| Authors: | Türeci, Ö; Chen, Y. T.; Sahin, U.; Güre, A. O.; Zwick, C.; Villena, C.; Tsang, S.; Seitz, G.; Old, L. J.; Pfreundschuh, M. |
| Article Title: | Expression of SSX genes in human tumors |
| Abstract: | The HOM-MEL-40 antigen which is encoded by the SSX-2 gene was originally detected as a tumor antigen recognized by autologous IgG antibodies in a melanoma patient. Expression analysis demonstrated that SSX-2 is a member of the recently described cancer/testis antigen (CTA) class as it is expressed in a variety of different human neoplasms, but not in normal tissues with the exception of testis and a weak expression in the thyroid. Further studies demonstrated that SSX-2 belongs to a gene family consisting of at least 5 homologous genes. We now report the analysis of the expression of all 5 SSX genes in 325 specimens of human neoplasms from various histological origins, using reverse transcription polymerase chain reaction (RT-PCR). SSX-1, -2, and -4 were found to be expressed in 8%, 15% and 15%, of the tumors, respectively, while the expression of the SSX-5 gene was rare (71325), and SSX-3 expression was not detected. For defined tumor types, expression of at least one of the SSX family members was most frequently observed in head and neck cancer (75%), followed by ovarian cancer (50%), malignant melanoma (43%), lymphoma (36%), colorectal cancer (27%) and breast cancer (23%), while leukemias and the few cases of leiomyosarcomas, seminomas and thyroid cancers were found not to express any SSX gene. |
| Keywords: | human tissue; polymerase chain reaction; neoplasms; colorectal cancer; melanoma; reverse transcription polymerase chain reaction; ovary cancer; gene expression; neoplasm proteins; tumor antigen; gene expression regulation, neoplastic; antigen presentation; immune response; antigens, neoplasm; head and neck cancer; lymphoma; thyroid cancer; leiomyosarcoma; repressor proteins; seminoma; multigene family; humans; human; priority journal; article |
| Journal Title: | International Journal of Cancer |
| Volume: | 77 |
| Issue: | 1 |
| ISSN: | 0020-7136 |
| Publisher: | John Wiley & Sons |
| Date Published: | 1998-07-03 |
| Start Page: | 19 |
| End Page: | 23 |
| Language: | English |
| DOI: | 10.1002/(sici)1097-0215(19980703)77:1<19::aid-ijc4>3.0.co;2-2 |
| PUBMED: | 9639388 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 12 December 2016 -- Source: Scopus |
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