Reciprocal recruitment of DRIP/mediator and p160 coactivator complexes in vivo by estrogen receptor Journal Article

  


Authors: Burakov, D.; Crofts, L. A.; Chang, C. P. B.; Freedman, L. P.
Article Title: Reciprocal recruitment of DRIP/mediator and p160 coactivator complexes in vivo by estrogen receptor
Abstract: Two functionally distinct classes of coactivators are recruited by liganded estrogen receptor, the DRIP/Mediator complex and p160 proteins, although the relative dynamics of recruitment is unclear. Previously, we have shown a direct, estradiol-dependent interaction between the DRIP205 subunit of the DRIP complex and the estrogen receptor (ER) AF2 domain. Here we demonstrate the in vivo recruitment of other endogenous DRIP subunits to ER in response to estradiol treatment in MCF-7 cells. To explore the relationship between DRIP and p160 coactivators, we examined the kinetics of coactivator recruitment to the ER target promoter, pS2, by chromatin immunoprecipitation. We observed a cyclic association and dissociation of coactivators with the promoter, with recruitment of p160s and DRIPs occurring in opposite phases, suggesting an exchange between these coactivator complexes at the target promoter.
Keywords: controlled study; unclassified drug; human cell; promoter region; proteins; protein protein interaction; protein; in vivo study; tumor cells, cultured; nuclear proteins; kinetics; reverse transcriptase polymerase chain reaction; ligand; immunoprecipitation; receptors, estrogen; trans-activators; protein subunit; estrogen receptor; estradiol; biochemistry; dissociation; hormones; protein p160; humans; human; priority journal; article; mink cell focus-forming virus; protein drip
Journal Title: Journal of Biological Chemistry
Volume: 277
Issue: 17
ISSN: 0021-9258
Publisher: American Society for Biochemistry and Molecular Biology  
Date Published: 2002-04-26
Start Page: 14359
End Page: 14362
Language: English
DOI: 10.1074/jbc.C200099200
PUBMED: 11893728
PROVIDER: scopus
DOI/URL:

http://www.scopus.com/inward/record.url?eid=2-s2.0-0037177857&partnerID=40&md5=536607e4382fe7efb2255d106cefc43b
Notes: Export Date: 14 November 2014 -- Source: Scopus
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MSK Authors
  1. Leonard P Freedman
    80 Freedman
  2. Linda A Crofts
    1 Crofts
  3. Chao-Pei Chang
    8 Chang
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