Two distinct coactivators, DRIP/mediator and SRC/p160, are differentially involved in VDR transactivation during keratinocyte differentiation Journal Article
| Authors: | Oda, Y.; Sihlbom, C.; Chalkley, R. J.; Huang, L.; Rachez, C.; Chang, C.; Burlingame, A. L.; Freedman, L. P.; Bikle, D. D. |
| Article Title: | Two distinct coactivators, DRIP/mediator and SRC/p160, are differentially involved in VDR transactivation during keratinocyte differentiation |
| Abstract: | Cell programs such as proliferation and differentiation involve the sequential activation and repression of gene expression. Vitamin D, via its active metabolite 1,25-dihydroxyvitamin D (1,25(OH) 2D 3), controls the proliferation and differentiation of a number of cell types, including keratinocytes, by directly regulating transcription. Two classes of coactivators, the Vitamin D receptor (VDR) interacting proteins (DRIP/mediator) and the p160 steroid receptor coactivator family (SRC/p160), control the actions of nuclear hormone receptors, including the Vitamin D receptor. However, the relationship between these two classes of coactivators is not clear. Using GST-VDR affinity beads, we have identified the DRIP/mediator complex as the major VDR binding complex in proliferating keratinocytes. After the cells differentiated, members of the SRC/p160 family were identified in the complex but not major DRIP subunits. Both DRIP205 and SRC-3 potentiated Vitamin D-induced transcription in proliferating cells, but during differentiation, DRIP205 was no longer effective. These results indicate that these two distinct coactivators are differentially involved in Vitamin D regulation of gene transcription during keratinocyte differentiation, suggesting that these coactivators are part of the means by which the temporal sequence of gene expression is regulated during the differentiation process. © 2004 Elsevier Ltd. All rights reserved. |
| Keywords: | controlled study; unclassified drug; human cell; conference paper; binding affinity; protein function; cell proliferation; animals; complex formation; gene expression; protein; cell differentiation; protein tyrosine kinase; proteomics; keratinocyte; cell type; transcription factors; nuclear proteins; gene expression regulation; transcription regulation; transactivation; transcription; rats; trans-activators; hormone receptor; vitamin d receptor; keratinocytes; spectrometry, mass, matrix-assisted laser desorption-ionization; mediator; coactivator; trans-activation (genetics); receptors, calcitriol; human; protein coactivator; protein p160 steroid receptor; vitamin d receptor interacting protein |
| Journal Title: | Journal of Steroid Biochemistry and Molecular Biology |
| Volume: | 89-90 |
| ISSN: | 0960-0760 |
| Publisher: | Pergamon-Elsevier Science Ltd |
| Date Published: | 2004-05-01 |
| Start Page: | 273 |
| End Page: | 276 |
| Language: | English |
| DOI: | 10.1016/j.jsbmb.2004.03.106 |
| PROVIDER: | scopus |
| PUBMED: | 15225784 |
| DOI/URL: | |
| Notes: | Presented at the 12th Workshop on Vitamin D; 2003 Jul 6-10; Maastricht, Netherlands -- Cited By (since 1996):11 -- Export Date: 16 June 2014 -- CODEN: JSBBE -- Source: Scopus |
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