β-pix directs collective migration of anterior visceral endoderm cells in the early mouse embryo Journal Article


Authors: Omelchenko, T.; Rabadan, M. A.; Hernandez-Martínez, R. ; Grego Bessa, J.; Anderson, K. V.; Hall, A.
Article Title: β-pix directs collective migration of anterior visceral endoderm cells in the early mouse embryo
Abstract: Collective epithelial migration is important throughout embryonic development. The underlying mechanisms are poorly understood but likely involve spatially localized activation of Rho GTPases. We previously reported that Rac1 is essential for generating the protrusive activity that drives the collective migration of anterior visceral endoderm (AVE) cells in the early mouse embryo. To identify potential regulators of Rac1, we first performed an RNAi screen of Rho family exchange factors (guanine nucleotide exchange factor [GEF]) in an in vitro collective epithelial migration assay and identified β-Pix. Genetic deletion of β-Pix in mice disrupts collective AVE migration, while high-resolution live imaging revealed that this is associated with randomly directed protrusive activity. We conclude that β-Pix controls the spatial localization of Rac1 activity to drive collective AVE migration at a critical stage in mouse development.
Keywords: mus; rho gtpases; mouse embryo; collective cell migration; anterior–posterior axis; ave migration; gefs
Journal Title: Genes and Development
Volume: 28
Issue: 24
ISSN: 0890-9369
Publisher: Cold Spring Harbor Laboratory Press  
Date Published: 2014-12-15
Start Page: 2764
End Page: 2777
Language: English
DOI: 10.1101/gad.251371.114
PROVIDER: scopus
PMCID: PMC4265679
PUBMED: 25512563
DOI/URL:
Notes: Export Date: 2 January 2015 -- Source: Scopus
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