β-Pix-dependent cellular protrusions propel collective mesoderm migration in the mouse embryo Journal Article

   


Authors: Omelchenko, T.; Hall, A.; Anderson, K. V.
Article Title: β-Pix-dependent cellular protrusions propel collective mesoderm migration in the mouse embryo
Abstract: Coordinated directional migration of cells in the mesoderm layer of the early embryo is essential for organization of the body plan. Here we show that mesoderm organization in mouse embryos depends on β-Pix (Arhgef7), a guanine nucleotide exchange factor for Rac1 and Cdc42. As early as E7.5, β-Pix mutants have an abnormally thick mesoderm layer; later, paraxial mesoderm fails to organize into somites. To define the mechanism of action of β-Pix in vivo, we optimize single-cell live-embryo imaging, cell tracking, and volumetric analysis of individual and groups of mesoderm cells. Use of these methods shows that wild-type cells move in the same direction as their neighbors, whereas adjacent β-Pix mutant cells move in random directions. Wild-type mesoderm cells have long polarized filopodia-like protrusions, which are absent in β-Pix mutants. The data indicate that β-Pix-dependent cellular protrusions drive and coordinate collective migration of the mesoderm in vivo. © 2020, The Author(s).
Keywords: genetics; mutation; phenotype; animal; cytology; metabolism; animals; embryo; green fluorescent protein; cell motion; embryology; morphogenesis; mice, inbred c57bl; c57bl mouse; imaging, three-dimensional; cell movement; green fluorescent proteins; cell polarity; embryo, mammalian; rodent; epithelium; mesoderm; flow kinetics; cell surface extensions; gastrulation; cell surface; cell; primitive streak; mammalian embryo; three-dimensional imaging; rheology; male; female; rho guanine nucleotide exchange factor; rho guanine nucleotide exchange factors
Journal Title: Nature Communications
Volume: 11
ISSN: 2041-1723
Publisher: Nature Publishing Group  
Date Published: 2020-11-27
Start Page: 6066
Language: English
DOI: 10.1038/s41467-020-19889-1
PUBMED: 33247143
PROVIDER: scopus
PMCID: PMC7695707
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-85096762282&doi=10.1038%2fs41467-020-19889-1&partnerID=40&md5=4eb855764a647cc037b8948be644003a
Notes: Article -- Export Date: 4 January 2021 -- Source: Scopus
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MSK Authors
  1. Kathryn Anderson
    148 Anderson
  2. Alan Hall
    41 Hall
  3. Tatiana A Omelchenko
    19 Omelchenko
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