Measuring recombination poficiency in mouse embryonic stem cells Journal Article
| Authors: | Pierce, A. J.; Jasin, M. |
| Article Title: | Measuring recombination poficiency in mouse embryonic stem cells |
| Abstract: | A method is presented to measure homologous recombination in mouse embryonic stem cells by both gene targeting and short-tract gene conversion of a double-strand break (DSB). A fluorescence-based reporter is first gene targeted to the Hprt locus in a quantifiable way. A homing endonuclease expression vector is then introduced to generate a DSB, the repair of which is also quantifiable. |
| Keywords: | flow cytometry; gene; gene conversion; dna; double-strand breaks; recombination; pathway; homology-directed repair; mammalian-cells; targeting; green fluorescent protein (gfp); double-strand break (dsb); hprt; mouse embryonic stem; (es) cells; i-scei; homing endonuclease |
| Journal Title: | Methods in Molecular Biology |
| Volume: | 1105 |
| ISSN: | 1064-3745 |
| Publisher: | Humana Press Inc |
| Date Published: | 2014-01-01 |
| Start Page: | 481 |
| End Page: | 495 |
| Language: | English |
| ACCESSION: | WOS:000333851000035 |
| PROVIDER: | wos |
| DOI: | 10.1007/978-1-62703-739-6_34 |
| Notes: | Chapter 34 in "Molecular Toxicology Protocols" (ISBN: 978-1-62703-738-9) -- Source: Wos |
