Gene editing 20 years later Journal Article
| Author: | Jasin, M. |
| Article Title: | Gene editing 20 years later |
| Abstract: | Directed modification of the genome is critical for interrogating gene function and can also be applied for gene therapy. Two decades ago a double-strand break (DSB) in the genome was discovered to induce efficient gene modification, either by homologous recombination with introduced DNA, i.e., gene targeting, or imprecise joining of DNA ends leading to mutagenesis. The accelerating development of technologies-meganucleases, ZFNs, TALENs, and CRISPR/Cas9- to introduce DSBs at specific sites in the genome for the purposes of modification is revolutionizing the biological and biomedical sciences. This chapter provides an overview of the research that led to these advances in gene editing and also summarizes DSB repair mechanisms in mammalian cells. |
| Keywords: | gene targeting; double-strand breaks; recombination; embryonic stem-cells; crystal-structure; double-strand; homologous; nonhomologous end-joining; homology-directed repair; mammalian-cells; binding domains; nhej; zinc-finger nucleases; break; cas9; genome engineering; gene editing; genome rearrangement; chromosomal translocation mechanisms; tal effectors; dna recognition |
| Journal Title: | Advances in Experimental Medicine and Biology |
| Volume: | 895 |
| ISSN: | 0065-2598 |
| Publisher: | Springer |
| Date Published: | 2016-03-04 |
| Start Page: | 1 |
| End Page: | 14 |
| Language: | English |
| ACCESSION: | WOS:000385740200002 |
| PROVIDER: | wos |
| DOI: | 10.1007/978-1-4939-3509-3_1 |
| Notes: | Article; Book Chapter 1 in "Genome Editing: The Next Step in Gene Therapy" (ISBN: 978-1-4939-3507-9) -- Source: Wos |
